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001-es BibID:	BIBFORM127814
Első szerző:	Cseh Zoltánné
Cím:	Effect of various sterilization procedures on the invitro germination of carnation species protected in Hungary / Zoltánné Cseh, Judit Dobránszki, Ivett Novák-Hermann, Pál Szarvas, Dóra Farkas, Angela Kolesznyk, Judit Csabai
Dátum:	2024
Megjegyzések:	In our research, we used plants of the family Caryophyllaceae (carnations), Dianthus plumarius subsp. praecox, Dianthus giganteiformis Borbás subsp. pontederae, and Dianthus superbus L. From a conservation point of view, all three species are important in Hungary, but the most outstanding is Dianthus plumarius subsp. praecox from Aggtelek karst and the Bükk region, which has a conservation value of 100 000 HUF, while the other two species have a value of 5000 HUF. Our aim was to develop a method of gene conservation, to investigate the possibilities of in vitro propagation in ex situ programs, and to perform further morphological and analytical analyses. In plant tissue culture, the bottleneck is surface sterilisation, one of the primary steps in in vitro work, and all subsequent steps depend on the effectiveness of this procedure. In the case of protected plants, in vitro culture should be started from seed to keep gene diversity as high as possible. The propagating material was obtained from the Botanical Garden collection. Different concentrations of detergents, different exposure times, and their combinations were used to start tissue culture. The optimal disinfectant/concentration/introduction time combination was revealed during the research to produce in vitro cultures of the three carnation species with the highest values for germination, purity, and growth.
ISBN:	978-615-6032-72-0
Tárgyszavak:	Természettudományok Biológiai tudományok előadáskivonat könyvrészlet Dianthus in vitro propagation gene preservation
Megjelenés:	Fenntartható Tápanyag-gazdálkodási Tudományos Műhely Konferenciája 2024 Innovatív megoldások a XXI. század mezőgazdaságában / szerk. Oláh Katalin, Kosztyuné Krajnyák Edit, Szabó Béla, Bara Eszter. - p. 180-186. -
További szerzők:	Dobránszki Judit (1967-) (biológus) Novák-Hermann Ivett Szarvas Pál (1977-) (molekuláris biológus) Farkas Dóra (1997-) (biomérnök) Kolesznyk, Angela Csabai Judit (1973-) (biológus, botanikus)
Internet cím:	Szerző által megadott URL Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM120356
Első szerző:	Cseh Zoltán
Cím:	Osoblivosti vvedennja v kul''turu in vitro ridkisnih taksoniv gvozdik Ugorshhini = The peculiarities of in vitro introduction of rare carnation taxa from Hungary / Cseh Z., Dobránszky J., Novák-Hermann I., Szarvas P., Farkas D., Csabai J., Kolesnyk A.
Dátum:	2023
Megjegyzések:	Cultivation of plants in vitro is currently one of the most common methods of vegetative propagation of plants. It makes it possible to obtain homogeneous planting material in large quantities within a short time, on small areas, and at relatively low cost; to preserve particularly valuable pheno- and genotypes, or to obtain biologically active substances of plant origin. The use of microclonal plant propagation is an excellent complement to the conservation of critical species that are endangered in their natural habitats or have difficult generative reproduction. The main goal of our research ?larification the peculiarities of tissue culture of three rare and endangered species of the Hungarian flora, viz. Dianthus plumarius ssp. praecox (Willd. ex Spreng.) Domin, Dianthus giganteiformis Borbás ssp. pontederae (Borbás) Soó, and Dianthus superbus L. Healthy and viable seeds were used as initial explants. The sterilising reagents used were different concentrations of mercuric hippochloride (HgCl2), sodium chloride (NaClO) and commercial chloramine B (NH2Cl). Subsequently, the plants were grown on Murashige and Skoog agarified hormone-free medium. It was established that for the seeds of the three researched carnation taxa, sterilization with 2,5% chloramine B for 15 minutes is the most optimal, this ensures obtaining aseptic planting material capable of intensive germination and formation of viable explants. For the induction of rhizogenesis, the most optimal is a modified MS medium with the addition of indolylacetic acid at a concentration of 0,1 mg/l.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk dianthus in vitro posztaszeptikus adaptáció mikroklonális szaporodás sterilizálás
Megjelenés:	Naukovij visnik Uzhgorodskogo Universitetu Serija Biologia = Scientific Bulletin Uzhgorod National University: Series Biology. - 54 (2023), p. 128-134. -
További szerzők:	Dobránszki Judit (1967-) (biológus) Novák-Hermann Ivett Szarvas Pál (1977-) (molekuláris biológus) Farkas Dóra (1997-) (biomérnök) Csabai Judit (1973-) (biológus, botanikus) Kolesnyk Angela
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