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001-es BibID:	BIBFORM117398
035-os BibID:	(cikkazonosító)05001 (Scopus)85181554728
Első szerző:	Fajardo, Roland
Cím:	Continental vs. tropical breed: Immunity comparison under heat stress conditions utilizing qRT-PCR technique / Roland Fajardo, Putri Kusuma Astuti, Zoltán Bagi, Lilla Bodrogi, Tímea Pintér, Gabriella Skoda, Husein Ohran, George Wanjala, Szilvia Kusza
Dátum:	2023
ISSN:	2273-1709 2117-4458
Megjegyzések:	Understanding the responses of animals to seasonal heat stress on the genomic level has led to the identification of genes implicated in thermal stress reaction mechanisms. In this study, the relative gene expression of Interleukin-10 (IL-10), an anti-inflammatory cytokine and biomarker for heat stress-mediated immune modulation, was observed during the summer and winter seasons in continental and tropical sheep breeds, namely, Hungarian Indigenous Tsigai and White Dorper, employing quantitative real-time polymerase chain reaction (qRT-PCR). Temperature-humidity indices (THI) were calculated to assess heat stress levels. The results indicate that IL-10 CT-values were significantly higher during the summer, when heat stress prevails, in both sheep breeds compared to winter. While the White Dorper exhibited a higher numerical value for the summer relative gene expression ratio (16.2) compared to the Hungarian Indigenous Tsigai (12.3), no significant differences in CT values were observed between breeds or among sexes. These findings suggest the immune-adaptive characteristics of the two sheep breeds during seasonal heat stress. The variation of IL-10 gene expression levels between the two breeds can be attributed to their geographical origins; the White Dorper emerging from arid subtropical South Africa and the Hungarian Indigenous Tsigai Sheep thriving in the seasonally harsh Carpathian climate for centuries.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok konferenciacikk folyóiratcikk gene expression heat stress Hungarian Tsigai IL-10 White Dorper
Megjelenés:	BIO Web of Conferences. - 8 (2023), p. 1-8. -
További szerzők:	Astuti, Putri Kusuma (1994-) (állattenyésztési genetikus) Bagi Zoltán (1987-) (természetvédelmi mérnök, állatgenetika) Bodrogi Lilla Pintér Tímea Skoda Gabriella Ohran, Husein Wanjala, George (1984-) (Animal scientist) Kusza Szilvia (1979-) (agrármérnök)
Pályázati támogatás:	2021-1.2.4-TÉT-2021-00047 Egyéb EFOP-3.6.2-16-2017-00001 EFOP
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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