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001-es BibID:	BIBFORM111442
035-os BibID:	(Scopus)85158924988 (WoS)000983708900001
Első szerző:	Astuti, Putri Kusuma (állattenyésztési genetikus)
Cím:	Genetic polymorphism in European and African sheep breeds reared in Hungary based on 48 SNPs associated with resistance to gastrointestinal parasite infection using KASP-PCR technique / Putri Kusuma Astuti; Dinu Gavojdian; Daniela Elena Ilie; George Wanjala; István Monori; Zoltán Bagi; Szilvia Kusza
Dátum:	2023
ISSN:	0049-4747 1573-7438
Megjegyzések:	This pilot study used an alternative and economically efcient technique, the Kompetitive Allele-Specifc Polymerase Chain Reaction (KASP-PCR) to examine 48 SNPs from 11 parasite-resistance genes found on 8 chromosomes in 110 animals from fve sheep breeds reared in Hungary; Hungarian Tsigai, White Dorper, Dorper, Ile de France, and Hungarian Merino. Allele and genotype frequencies, fxation index, observed heterozygosity, expected heterozygosity, F statistic, and their relationship with the Hardy-Weinberg equilibrium (WHE) and the polymorphic information content (PIC) were determined, followed by principal component analysis (PCA). As much as 32 SNPs out of the 48 initially studied were successfully genotyped. A total of 9 SNPs, 4 SNPs in TLR5, 1 SNP in TLR8, and 4 SNPs in TLR2 genes, were polymorphic. The variable genotype and allele frequency of the TLRs gene indicated genetic variability among the studied sheep breeds, with the Hungarian Merino exhibiting the most polymorphisms, while Dorper was the population with the most SNPs departing from the HWE. According to the PIC value, the rs430457884-TLR2, rs55631273-TLR2, and rs416833129-TLR5 were found to be informative in detecting polymorphisms among individuals within the populations, whereas the rs429546187-TLR5 and rs424975389- TLR5 were found to have a signifcant infuence in clustering the population studied. This study reported a moderate level of genetic variability and that a low to moderate within-breed diversity was maintained in the studied populations.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk KASP PCR Ovis aries Parasite resistance genes Polymorphism TLRs
Megjelenés:	Tropical Animal Health and Production. - 55 : 3 (2023), p. 1-12. -
További szerzők:	Gavojdian, Dinu Ilie, Daniela Elena Wanjala, George (1984-) (Animal scientist) Monori István (1976-) (agrármérnök) Bagi Zoltán (1987-) (természetvédelmi mérnök, állatgenetika) Kusza Szilvia (1979-) (agrármérnök)
Pályázati támogatás:	EFOP-3.6.2-16-2017-00001 EFOP VP3-16.1.1-4.1.5- 4.2.1-4.2.2-8.1.1-8.2.1-8.3.1-8.5.1-8.5.2-8.6.1-17 Egyéb
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001-es BibID:	BIBFORM106134
035-os BibID:	(WOS)000904416000001 (Scopus)85144635665
Első szerző:	Astuti, Putri Kusuma (állattenyésztési genetikus)
Cím:	Validation of SNP markers for thermotolerance adaptation in Ovis aries adapted to different climatic regions using KASP-PCR technique / Putri Kusuma Astuti, Daniela Elena Ilie, Dinu Gavojdian, George Wanjala, Bouabid Badaoui, Husein Ohran, Eva Pasic-Juhas, Zoltán Bagi, András Jávor, Szilvia Kusza
Dátum:	2022
ISSN:	2045-2322
Megjegyzések:	A study on 51 SNPs belonging to 29 genes related to heat stress was carried out in 720 sheep from 17 different breeds adapted to different climates from Hungary, Bosnia and Herzegovina, Morocco and Romania, using Kompetitive Allele?Specific Polymerase Chain Reaction. Genotype frequency and the Hardy?Weinberg equilibrium were calculated, followed by a clustering using the Principal Component Analysis. We analyzed the polymorphisms in the following genes analyzed: HSPA12A, HSP90AA1, IL33, DIO2, BTNL2, CSN2, ABCG1, CSN1S1, GHR, HSPA8, STAT3, and HCRT. We emphasized on HSPA12A and HSPA8 genes as they were successfully genotyped in all studied flocks in which genotype frequency patterns were identified. Contrary to previous findings, the A allele for HSPA8 SNP was not observed in the heat tolerant breeds, being found exclusively in cold-tolerant breeds. The principal component analysis could not clearly differentiate the breeds, while plot concentration was slightly varied among the three groups, with HSP90AA1 and IL33 SNPs' loading values significantly contributing to PC1 and PC2. We confirmed previous works that the HSPA12A, HSPA8, HSP90AA1 and IL33 SNPs are potential candidate markers for thermotolerance adaptation in sheep. This research contributes to the genetic variability of SNPs for thermotolerance adaptability in sheep.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Scientific Reports. - 12 (2022), p. 1-9. -
További szerzők:	Ilie, Daniela Elena Gavojdian, Dinu Wanjala, George (1984-) (Animal scientist) Badaoui, Bouabid (1979-) (animal genetics engineer) Ohran, Husein Pasic-Juhas, Eva Bagi Zoltán (1987-) (természetvédelmi mérnök, állatgenetika) Jávor András (1952-) (agrármérnök) Kusza Szilvia (1979-) (agrármérnök)
Pályázati támogatás:	EFOP-3.6.2-16-2017-00001 EFOP 2021-1.2.4-TÉT-2021-00014 Egyéb
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001-es BibID:	BIBFORM129638
035-os BibID:	(Scopus)105003158632 (WoS)001473869700019
Első szerző:	Wanjala, George (Animal scientist)
Cím:	Genetic diversity and adaptability of native sheep breeds from different climatic zones / George Wanjala, Zoltán Bagi, Dinu Gavojdian, Bouabid Badaoui, Putri Kusuma Astuti, Alexandru Mizeranschi, Elena Ilisiu, Husein Ohran, Eva Pasic Juhas, Dimitrios Loukovitis, Aldona Kawȩcka, Rūta Šveistienẻ, Zsolt Becskei, Péter Strausz, Nelly Kichamu, Szilvia Kusza
Dátum:	2025
ISSN:	2045-2322
Megjegyzések:	To better understand the genetic architecture and adaptability of native sheep, 22 breeds were genotyped using ovine50K SNP chips. Eleven additional populations from open-source repositories were included. Cross population Extended Haplotype Homozygosity (XP-EHH) and Relative Scaled Haplotype Homozygosity (Rsb) haplotype-based approaches were used to identify genetic variations influencing the adaptation of local sheep breeds to different climatic zones. The results indicate that all breeds exhibited high but declining genetic diversity levels, with a larger proportion of genetic variation explained by development history and refined by geographical origin, as shown by principal component analysis and Neighbor Net graphs. Admixture analysis revealed high admixture levels in European and North African breeds. Using XP-EHH and Rsb methods, 371 genes were putatively under selection, with only nine common among all population pairs, highlighting unique adaptability. Most identified genes, including Interleukin (IL) and cluster differentiation (CD) gene families underlie immune responses, emphasizing their role in resilience to climate change effects. These findings support that indigenous sheep breeds have retained significant genetic diversity, but improper genetic management may threaten it. Additionally, the research emphasizes that indigenous sheep breeds' unique adaptability to specific climatic zones makes them valuable genetic assets for developing climate-resilient breeds.
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	Scientific Reports. - 15 : 14143 (2025), p. 1-13. -
További szerzők:	Bagi Zoltán (1987-) (természetvédelmi mérnök, állatgenetika) Gavojdian, Dinu Badaoui, Bouabid (1979-) (animal genetics engineer) Astuti, Putri Kusuma (1994-) (állattenyésztési genetikus) Mizeranschi, Alexandru Eugeniu Ilisiu, Elena Ohran, Husein Pasic Juhas, Eva Loukovitis, Dimitrios Kawęcka, Aldona Šveistienẻ, Rūta Becskei Zsolt Strausz Péter Kichamu, Nelly (1978-) (Animal scientist) Kusza Szilvia (1979-) (agrármérnök)
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001-es BibID:	BIBFORM105933
Első szerző:	Wanjala, George (Animal scientist)
Cím:	Genomic diversity and population structure of Hungarian and Romanian native sheep breeds / George Wanjala, Elena Ilisiu, Dinu Gavojdian, Zoltán Bagi, Putri Kusuma Astuti, Kusza Szilvia
Dátum:	2022
Tárgyszavak:	Agrártudományok Állattenyésztési tudományok előadáskivonat könyvrészlet
Megjelenés:	Biotechnology at the University of Debrecen - 2022 International Symposium : Abstract Book. - p 68-69. -
További szerzők:	Ilisiu, Elena Gavojdian, Dinu Bagi Zoltán (1987-) (természetvédelmi mérnök, állatgenetika) Astuti, Putri Kusuma (1994-) (állattenyésztési genetikus) Kusza Szilvia (1979-) (agrármérnök)
Pályázati támogatás:	2021-1.2.4-TÉT-2021-00014 Egyéb
Internet cím:	Intézményi repozitóriumban (DEA) tárolt változat
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