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001-es BibID:	BIBFORM131144
035-os BibID:	(wos)001528019400001 (Scopus)105011040443
Első szerző:	Ahmad, Hala
Cím:	Retinoic acid differently modulates NOD1/NOD2-mediated inflammatory responses in human macrophage subsets / Ahmad Hala, Alatshan Ahmad, Bíró Eduárd, Benkő Szilvia
Dátum:	2025
ISSN:	1664-3224
Megjegyzések:	Macrophages are indispensable in homeostasis and innate immune responses in multiple tissues, while their polarization and functional characteristics are determined by the activating stimuli and their tissue microenvironment. The vitamin A derivative retinoic acid shows inhomogeneous distribution among the tissues and has an important modulatory role in inflammatory responses. However, its effects on the cytokine secretion induced by the cytosolic pattern-recognition receptors NOD1 and NOD2 are unclear. In our study, we used human monocyte-derived macrophages differentiated in the presence of GM-CSF or M-CSF to generate inflammation inducing (GM-M?) or inflammation resolving (M-M?) cells, respectively. We activated the cells with either a NOD1- or NOD2 specific agonist and, using ELISA, we determined the pattern and dynamics of cytokines secreted by the macrophage subpopulations. Furthermore, we studied the effect of all-trans retinoic acid (ATRA) pre-treatment on the NOD1- and NOD2-induced cytokine release. Our comparative analysis shows subpopulation-characteristic pattern of cytokine secretion, as GM-M? produce significantly higher pro-inflammatory IL-6, IL-8, TNF-? and IL-1?, while M-M? secret higher anti-inflammatory IL-10. However, IL-18 and IFN? secretion was comparable between the M? subpopulations. We also show for the first time that ATRA has marked impact on cytokine secretion triggered by NOD1 and NOD2. Importantly however, the ATRA-induced changes of cytokine secretion follow opposite tendency in two M? subpopulations. In conclusion, these results show that NOD1/NOD2-induced cytokine secretion by macrophage subsets is highly context-dependent and our results highlight the importance of the retinoic acid content of the local tissue environment in shaping macrophage function in health and disease.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk retinoic acid NOD1 NOD2 NOD-like receptor vitamin A inflammation macrophage cytokine
Megjelenés:	Frontiers in Immunology. - 16 (2025), p. 1-11. -
További szerzők:	Alatshan, Ahmad (1984-) (immunológus) Bíró Eduárd (1998-) (molekuláris biológus) Benkő Szilvia (1973-) (molekuláris biológus)
Pályázati támogatás:	K131844 OTKA K147109 OTKA Richter Talentum Foundation Egyéb Stipendium Hungaricum Scholarship Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM096580
035-os BibID:	(WoS)000676356300001 (Scopus)85109904667
Első szerző:	Kovács Elek Gergő
Cím:	Caffeine Has Different Immunomodulatory Effect on the Cytokine Expression and NLRP3 Inflammasome Function in Various Human Macrophage Subpopulations / Elek Gergő Kovács, Ahmad Alatshan, Marietta Margit Budai, Zsolt Czimmerer, Eduárd Bíró, Szilvia Benkő
Dátum:	2021
ISSN:	2072-6643
Megjegyzések:	Besides its well-known psychoactive effects, caffeine has a broad range of actions. It regulates several physiological mechanisms as well as modulates both native and adaptive immune responses by various ways. Although caffeine is assumed to be a negative regulator of inflammation, the effect on the secretion of pro- and anti-inflammatory cytokines is highly controversial. Macrophages are major mediators of inflammatory responses; however, the various subpopulations develop different effects ranging from the initiation to the resolution of inflammation. Here we report a comparative analysis of the effect of caffeine on two subpopulations of human monocyte-derived macrophages differentiated in the presence of macrophage colony-stimulating factor (M-CSF) or granulocyte-macrophage colony-stimulating factor (GM-CSF), resulting in M-M?s and GM-M?s, respectively. We showed that although TNF-? secretion was downregulated in both LPS-activated M? subtypes by caffeine, the secretion of IL-8, IL-6, and IL-1? as well as the expression of Nod-like receptors was enhanced in M-M?s, while it did not change in GM-M?s. We showed that caffeine (1) altered adenosine receptor expression, (2) changed Akt/AMPK/mTOR signaling pathways, and (3) inhibited STAT1/IL-10 signaling axis in M-M?s. We hypothesized that these alterations play an important modulatory role in the upregulation of NLRP3 inflammasome-mediated IL-1? secretion in LPS-activated M-M?s following caffeine treatment.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk caffeine inflammation macrophages NLRP3 inflammasome cytokines signaling
Megjelenés:	Nutrients. - 13 : 7 (2021), p. 2409. -
További szerzők:	Alatshan, Ahmad (1984-) (immunológus) Budai Marietta Margit (1985-) (molekuláris biológus) Czimmerer Zsolt (1981-) (molekuláris biológus) Bíró Eduárd (1998-) (molekuláris biológus) Benkő Szilvia (1973-) (molekuláris biológus)
Pályázati támogatás:	K131844 OTKA FK132185 OTKA GINOP-2.3.2-15-2016-00040 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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