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001-es BibID:BIBFORM105403
Első szerző:Kocsis Beatrix (molekuláris biológus)
Cím:Study on a gene deletion mutant encoding a transcription factor regulating a secondary metabolite gene cluster / Beatrix Kocsis, Petra Fodor, Éva Julianna Leiter, István Pócsi
Megjegyzések:Whole genome sequencing and annotation projects of filamentous fungi in the last decade revealed numerous secondary metabolite gene clusters being silent under normal laboratory conditions. These gene clusters can be activated under environmental including oxidative stress and armour fungi with effective chemicals in competitive habitats. These chemicals can be exploited as bioactive agents like antibiotics, antimycotics, immunsuppresants for medical purposes. For example in the filamentous fungus model organism Aspergillus nidulans a non-ribosomal peptide synthase gene cluster including the synthase (ANID_07884) and its transcription factor (ANID_07872) activated under menadione sodium bisulfite treatment. In this study we generated the gene deletion mutant of ANID_07872 [1] and characterized the mutant phenotypically. The ?ANID_07872 gene deletion strain possessed higher sensitivity to oxidative stress inducing agents like diamide, tert-butyl hydroperoxide (tBOOH) compared to the control strain. Surprisingly the menadion sodium bisulfite sensitivity of the mutant and control strain was comparable. Secondary metabolite production of strains was also monitored in Aspergillus minimal medium supplemented with 2% maltose and 1% mycological peptone at 25?. A red colour UV active band from the mycelia of the mutant was detected using thin layer chromatography which was absent in the control strain [2]. Determination of this chemical is in progress and hopefully new metabolite will be found with bioactive properties.
Tárgyszavak:Természettudományok Biológiai tudományok előadáskivonat
Megjelenés:Abstracts of the 18th International Congress of the Hungarian Society for Microbiology. Acta Microbiologica et Immunologica Hungarica. - 66 : Suppl. 2 (2019), p. 155. -
További szerzők:Fodor Petra (1994-) (gyógyszer-biotechnológus) Leiter Éva (1976-) (biológus) Pócsi István (1961-) (vegyész)
Internet cím:DOI
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