CCL

Összesen 6 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM128863
035-os BibID:(scopus)86000593579 (wos)001445681200001
Első szerző:Farkas Anna (molekuláris biológus)
Cím:Potential Glycobiomarkers in Maternal Obesity and Gestational Diabetes During Human Pregnancy / Farkas Anna, Suranyi Andrea, Kolcsar Balint, Gyurkovits Zita, Kozinszky Zoltan, Vari Sandor G., Guttman Andras
Dátum:2025
ISSN:2077-0383
Megjegyzések:Introduction: Obesity is a rapidly growing common health problem worldwide that can lead to the development of gestational diabetes mellitus (GDM). However, GDM not only affects women with obesity but can also develop at any time, even after the OGTT test; therefore, an increasing number of complications related to GDM can be seen in both mothers and their children. It is necessary to discover biomarkers capable of indicating the development of GDM or complications during/after pregnancy. Since the N-glycosylation motif of human IgG has been described to change under many physiological and pathological conditions, it is a promising target for biomarker research. In our study, the effects of obesity and GDM were investigated on human serum IgG N-linked glycosylation patterns during human pregnancy. Materials and Methods: The study participants were categorized into four groups according to their body mass index (BMI) and GDM status: normal weight as control, obese (BMI > 30 kg/m2), normal weight with GDM, and obese with GDM. The released N-glycan components of IgG were separated with capillary electrophoresis and detected using a laser-induced fluorescence detector. Results: The result revealed several differences between the N-glycosylation patterns of the four study groups. Of this, 17 of the 20 identified structures differed significantly between the groups. The ratios of sialylated to non-sialylated structures were not changed significantly, but the core fucosylation level showed a significant decrease in the GDM and obese GDM groups compared to the control subjects. The lowest degree of core fucosylation was observed in the GDM group. Conclusions: The findings indicate that obesity in isolation does not have a significant impact on the IgG N-glycosylation pattern in pregnancy. Conversely, alterations in the N-glycan profile of antibodies may serve as biomarkers for the diagnosis of GDM in mothers with a normal BMI, although more evidence is needed. By incorporating glycan-based biomarkers into clinical practice, healthcare providers can improve early detection, personalize management strategies, and potentially mitigate adverse pregnancy outcomes associated with obesity and GDM.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Clinical Medicine. - 14 : 5 (2025), p. 1-15. -
További szerzők:Surányi Adnrea Kolcsár Bálint Gyurkovits Zita Kozinszky Zoltán Vári Sándor G. Guttman András (1954-) (vegyészmérnök)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM091997
Első szerző:Farkas Anna (molekuláris biológus)
Cím:Modeling of the Desialylated Human Serum N-glycome for Molecular Diagnostic Applications in Inflammatory and Malignant Lung Diseases / Anna Farkas, Brigitta Mészáros, Máté Szarka, Márton Szigeti, János Kappelmayer, Miklós Szabó, Eszter Csánky, András Guttman
Dátum:2020
ISSN:1566-5240
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Current Molecular Medicine. - 20 : 10 (2020), p. 765-772. -
További szerzők:Mészáros Brigitta (1990-) (vegyészmérnök) Szarka Máté (1990-) (biotechnológus) Szigeti Márton (1986-) (környezetmérnök) Kappelmayer János (1960-) (laboratóriumi szakorvos) Szabó Miklós (1980-) (tüdőgyógyász) Csánky Eszter (1959-) (tüdőgyógyász, klinikai immunológus, allergológus) Guttman András (1954-) (vegyészmérnök)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

3.

001-es BibID:BIBFORM121603
035-os BibID:(Scopus)85194393047 (WoS)001248542600001
Első szerző:Farsang Róbert
Cím:Glucose unit computation in capillary zone electrophoresis of carbohydrates using a numerical approximation based search for a virtual EOF marker. A tutorial / Robert Farsang, Anna Farkas, Gábor Jarvas, András Guttman
Dátum:2024
ISSN:0165-9936
Megjegyzések:Several high-resolution carbohydrate separation methods are currently available for structural elucidation in the analytical glycomics field, but the associated glyco-informatics support is lagging. Identifying protein-bound glycan structures represents a multifaceted challenge with elements of separation science, bioinformatics, and sophisticated computational methods. In electroosmotic flow (EOF) driven separations with counter currently migrating sample components, the apparent migration times of the analyte molecules are the result of the interplay between their effective electrophoretic mobilities and the EOF. The resulting continuously increasing migration time distances between the consecutively migrating maltodextrin oligomers make glucose unit value calculation and the subsequent structural interpretation of unknown glycans extremely challenging. To correct this irregularity, a numerical approximation-based search-derived virtual electroosmotic flow marker is introduced and utilized. This tutorial guides the reader through the computation process for structural elucidation of N-linked carbohydrates by using a detailed worked example with a monoclonal antibody of biopharmaceutical interest.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Carbohydrates
Capillary electrophoresis
Virtual EOF marker
Numerical approximation
Maltodextrin ladder
Glucose unit
Megjelenés:Trac-Trends In Analytical Chemistry. - 176 (2024), p. 1-9. -
További szerzők:Farkas Anna (1988-) (molekuláris biológus) Járvás Gábor (1982-) (vegyészmérnök) Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:2023-1.2.1-ERA_NET-2023-00015
Egyéb
1G3DBK0CTUF247
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

4.

001-es BibID:BIBFORM083098
035-os BibID:(WoS)000509616900017 (Scopus)85076839168
Első szerző:Mészáros Brigitta (vegyészmérnök)
Cím:Comparative analysis of the human serum N-glycome in lung cancer, COPD and their comorbidity using capillary electrophoresis / Brigitta Mészáros, Gábor Járvás, Anna Farkas, Márton Szigeti, Zsuzsanna Kovács, Renáta Kun, Miklós Szabó, Eszter Csánky, András Guttman
Dátum:2020
ISSN:1570-0232
Megjegyzések:Lung cancer (LC) and chronic obstructive pulmonary disease (COPD) are prevalent ailments with a great challenge to distinguish them based on symptoms only. Since they require different treatments, it is important to find non-invasive methods capable to readily diagnose them. Moreover, COPD increases the risk of lung cancer development, leading to their comorbidity. In this pilot study the N-glycosylation profile of pooled human serum samples (90 patients each) from lung cancer, COPD and comorbidity (LC with COPD) patients were investigated in comparison to healthy individuals (control) by capillary gel electrophoresis with high sensitivity laser-induced fluorescence detection. Sample preparation was optimized for human serum samples introducing a new temperature adjusted denaturation protocol to prevent precipitation and increased endoglycosidase digestion time to assure complete removal of the N-linked carbohydrates. The reproducibility of the optimized method was<3.5%. Sixty-one N-glycan structures were identified in the pooled control human serum sample and the profile was compared to pooled lung cancer, COPD and comorbidity of COPD with lung cancer patient samples. One important finding was that no other sugar structures were detected in any of the patient groups, only quantitative differences were observed. Based on this comparative exercise, a panel of 13 N-glycan structures were identified as potential glycobiomarkers to reveal significant changes (> 33% in relative peak areas) between the pathological and control samples. In addition to N-glycan profile changes, alterations in the individual N-glycan subclasses, such as total fucosylation, degree of sialylation and branching may also hold important glycobiomarker values.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Journal of Chromatography B-Analytical Technologies In The Biomedical And Life Sciences. - 1137 (2020), p. 1-7. -
További szerzők:Járvás Gábor (1982-) (vegyészmérnök) Farkas Anna (1988-) (molekuláris biológus) Szigeti Márton (1986-) (környezetmérnök) Kovács Zsuzsanna (1989-) Kun Renáta (1986-) (biotechnológus) Szabó Miklós (1980-) (tüdőgyógyász) Csánky Eszter (1959-) (tüdőgyógyász, klinikai immunológus, allergológus) Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:K 116263
NKFIH
BIONANO_GINOP-2.3.2-15-2016-00017
GINOP
NN 127062
NKFIH
ÚNKP-18-3-I-DE-393
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

5.

001-es BibID:BIBFORM130039
035-os BibID:(scopus)105004261149 (wos)001490430400001
Első szerző:Sárközy Dániel (PhD hallgató)
Cím:Rapid baseline hump-free analysis of therapeutic proteins in a wide molecular weight range by SDS - capillary agarose gel electrophoresis / Sárközy Dániel, Farkas Anna, Guttman András
Dátum:2025
ISSN:0003-2670
Megjegyzések:Background: Sodium dodecyl sulfate capillary gel electrophoresis traditionally employs entangled polymer networks (CE-SDS) or borate cross-linked gels (SDS-CGE) for size-based protein analysis. However, the separation of SDS proteins in these transiently cross-linked sieving matrices requires long analysis times and in addition, baseline humps/waves frequently occur making peak identification and quantification challenging. The analytical biopharma community has been trying to solve this problem for a decade, making it clear that a novel gel composition was required to provide baseline hump-free separation of SDS-proteins by capillary electrophoresis of higher MW biopharmaceuticals. Results: Using a transiently cross-linked agarose matrix shown in this paper enabled rapid separation of therapeutic proteins with excellent resolution, but more importantly, eliminated the commonly observed baseline disturbances of dextran-based gel formulations. Using capillaries as short as 10 cm effective length, the tetrahydroxyborate cross-linked agarose matrix supported fast analysis (~5 min) of an intact anti-SARS-CoV-2 therapeutic antibody and its subunits with excellent run-to-run migration time (RSD <0.3 %) and peak area (RSD <5 %) reproducibility. High resolution between the closely migrating non-glycosylated heavy chain and the heavy chain fragments was also obtained (RS = 1.65). The high molecular weight thyroglobulin (660 kDa), and the highly glycosylated fusion protein of etanercept were also successfully analyzed with borate-agarosebased gels, exploiting the stable baseline at the upper molecular weight range of the separation trace.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Capillary gel electrophoresis
Agarose
Sodium dodecyl sulfate
Therapeutic proteins
Baseline hump
Megjelenés:Analytica Chimica Acta. - 1360 (2025), p. 1-5. -
További szerzők:Farkas Anna (1988-) (molekuláris biológus) Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:NKFIH ADVANCED 150780
Egyéb
DE Publikációs Tudománytámogatási Program
Egyéb
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

6.

001-es BibID:BIBFORM092960
Első szerző:Török Rebeka
Cím:Evaluation of Possible Processing Time Effects on the Global N-Glycosylation Profile of Human Blood Samples / Rebeka Torok, Anna Farkas, András Guttman, Gabor Jarvas
Dátum:2020
ISSN:1566-5240
Megjegyzések:The utilization of N-glycan profiling recently gained high importance in fundamental biomedical and applied clinical research. However, for the time being, no glycan biomarker has been approved for clinical diagnosis by the regulatory agencies due to the lack of verifications on large patient cohorts and suitable analytical technologies. In this paper, the effect of human blood sample handling was studied prior to N-glycosylation profiling by capillary electrophoresis, coupled with high sensitivity fluorescence detection. Special attention was paid to the preservation of sialylated structures because of their important clinical - biological relevance. Our results suggested that it is adequate to refrigerate and store the collected total blood samples prior to analysis to obtain unbiased results. Furthermore, we report on the good practice of serum sample handling in order to prevent decomposition of the sialylated structures. Our findings may promote procedure standardization and easier clinical translation of diagnostic N-glycosylation profiling in molecular medicinal applications.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Current Molecular Medicine. - 20 : 10 (2020), p. 840-846. -
További szerzők:Farkas Anna (1988-) (molekuláris biológus) Guttman András (1954-) (vegyészmérnök) Járvás Gábor (1982-) (vegyészmérnök)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1 
Corvina könyvtári katalógus v10.1.21-SNAPSHOT © 2024 Monguz kft. Minden jog fenntartva.