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001-es BibID:	BIBFORM121603
035-os BibID:	(Scopus)85194393047 (WoS)001248542600001
Első szerző:	Farsang Róbert
Cím:	Glucose unit computation in capillary zone electrophoresis of carbohydrates using a numerical approximation based search for a virtual EOF marker. A tutorial / Robert Farsang, Anna Farkas, Gábor Jarvas, András Guttman
Dátum:	2024
ISSN:	0165-9936
Megjegyzések:	Several high-resolution carbohydrate separation methods are currently available for structural elucidation in the analytical glycomics field, but the associated glyco-informatics support is lagging. Identifying protein-bound glycan structures represents a multifaceted challenge with elements of separation science, bioinformatics, and sophisticated computational methods. In electroosmotic flow (EOF) driven separations with counter currently migrating sample components, the apparent migration times of the analyte molecules are the result of the interplay between their effective electrophoretic mobilities and the EOF. The resulting continuously increasing migration time distances between the consecutively migrating maltodextrin oligomers make glucose unit value calculation and the subsequent structural interpretation of unknown glycans extremely challenging. To correct this irregularity, a numerical approximation-based search-derived virtual electroosmotic flow marker is introduced and utilized. This tutorial guides the reader through the computation process for structural elucidation of N-linked carbohydrates by using a detailed worked example with a monoclonal antibody of biopharmaceutical interest.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Carbohydrates Capillary electrophoresis Virtual EOF marker Numerical approximation Maltodextrin ladder Glucose unit
Megjelenés:	Trac-Trends In Analytical Chemistry. - 176 (2024), p. 1-9. -
További szerzők:	Farkas Anna (1988-) (molekuláris biológus) Járvás Gábor (1982-) (vegyészmérnök) Guttman András (1954-) (vegyészmérnök)
Pályázati támogatás:	2023-1.2.1-ERA_NET-2023-00015 Egyéb 1G3DBK0CTUF247 Egyéb
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