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001-es BibID:	BIBFORM118556
035-os BibID:	(WoS)001184297100001 (Scopus)85184742360
Első szerző:	Tánczos Bence (biológus)
Cím:	Effects of H2S-donor ascorbic acid derivative and ischemia/reperfusion-induced injury in isolated rat hearts / Bence Tánczos, Virág Vass, Erzsébet Szabó, Miklós Lovas, Rasha Ghanem Kattoub, Ilona Bereczki, Anikó Borbás, Pál Herczegh, Árpád Tósaki
Dátum:	2024
ISSN:	0928-0987
Megjegyzések:	Hydrogen sulfide (H2S), a gasotransmitter, plays a crucial role in vasorelaxation, anti-inflammatory processes and mitigating myocardial ischemia/reperfusion-induced injury by regulating various signaling processes. We designed a water soluble H2S-releasing ascorbic acid derivative, BM-164, to combine the beneficial cardiovascular and anti-inflammatory effects of H2S with the excellent water solubility and antioxidant properties of ascorbic acid. DPPH antioxidant assay revealed that the antioxidant activity of BM-164 in the presence of a myocardial tissue homogenate (extract) increased continuously over the 120 min test interval due to the continuous release of H2S from BM-164. The cytotoxicity of BM-164 was tested by MTT assay on H9c2 cells, which resulted in no cytotoxic effect at concentrations of 10 to 30 mu M. The possible beneficial effects of BM-164 (30 mu M) was examined in isolated 'Langendorff' rat hearts. The incidence of ventricular fibrillation (VF) was significantly reduced from its control value of 79 % to 31 % in the BM-164 treated group, and the infarct size was also diminished from the control value of 28 % to 14 % in the BM-164 treated group. However, coronary flow (CF) and heart rate (HR) values in the BM-164 treated group did not show significantly different levels in comparison with the drug-free control, although a non-significant recovery in both CF and HR was observed at each time point. We attempted to reveal the mechanism of action of BM-164, focusing on the processes of autophagy and apoptosis. The expression of key autophagic and apoptotic markers in isolated rat hearts were detected by Western blot analysis. All the examined autophagy-related proteins showed increased expression levels in the BM-164 treated group in comparison to the drug-free control and/or ascorbic acid treated groups, while the changes in the expression of apoptotic markers were not obvious. In conclusion, the designed water soluble H2S releasing ascorbic acid derivative, BM-164, showed better cardiac protection against ischemia/ reperfusion-induced injury compared to the untreated and ascorbic acid treated hearts, respectively.
Tárgyszavak:	Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	European Journal Of Pharmaceutical Sciences. - 195 (2024), p. 1-15. -
További szerzők:	Vass Virág (1986-) (biológus) Szabó Erzsébet (1973-) (molekuláris biológus) Lovas Miklós (2000-) (gyógyszerészhallgató) Kattoub, Rasha Ghanem (1994-) Bereczki Ilona (1981-) (vegyész, antibiotikumkémikus) Borbás Anikó (1965-) (vegyész) Herczegh Pál (1947-) (vegyész, antibiotikumkémikus) Tósaki Árpád (1958-) (kísérletes farmakológus, gyógyszerész)
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001-es BibID:	BIBFORM124631
035-os BibID:	(scopus)85205365749
Első szerző:	Tósaki Ágnes (bőrgyógyász)
Cím:	A new cannabigerol derivative, LE-127/2, induces autophagy mediated cell death in human cutaneous melanoma cells / Tósaki Ágnes, Szabó Zsuzsanna, Király József, Lőrincz Eszter Boglárka, Vass Virág, Tánczos Bence, Bereczki Ilona, Herczegh Pál, Remenyik Éva, Tósaki Árpád, Szabó Erzsébet
Dátum:	2024
ISSN:	0928-0987
Megjegyzések:	Despite the targeted- and immunotherapies used in the past decade, survival rate among patients with metastatic melanoma remains low, therefore, melanoma is responsible for the majority of skin cancer-related deaths. The ongoing investigation of natural antitumor agents, the nonpsychoactive cannabinoid, cannabigerol (CBG) found in Cannabis sativa is emerging as a promising candidate. CBG offers a potential therapeutic role in the treatment of melanoma demonstrating cell growth inhibition in some tumors. Its low water solubility and bioavailability hinder the potential effectiveness. To address these challenges, a modified CBG, namely LE-127/2 was synthesized by Mannich-type reaction. The aim was to investigate the effect of this novel compound on cell proliferation as well as the mechanism of cell death with a particular focus on autophagy and apoptosis. Human cutan melanoma cell lines, WM35, A2058 and WM3000 were utilized for the present study. Cell proliferation of the cells after the treatment with LE-127/2, parent CBG or vemurafenib was assessed by Cell Titer Blue Assay. Cells were treated with a 1.25?80 ?M of the above-mentioned compounds, and it was found that at 20 ?M of all drugs showed a comparable effective inhibition of cell proliferation, however, vemurafenib and CBG proved to be more effective than LE-127/2. In addition, clonogenic cell survival assays were performed to examine the inhibitory effect of LE-127/2 on the colony formation ability of melanoma cell lines. Cells treated with 20 ?M of LE-127/2 for 14 days showed about a 50% suppression of clonogenic cell survival. LE-127/2 exerted the most intensive inhibition on A2058 cell colonies. Furthermore, notably, LDH cytotoxicity assay performed on HaCaT cell line, proved LE-127/2 to be cytotoxic only at higher concentration, such as 80 ?M, while the parent CBG was cytotoxic at concentration as low as 5 ?M, suggesting that the new CBG derivative as a drug candidate may be applied in human pharmacotherapy without causing a substantial damage in intact epidermal cells. Analysis of protein expression revealed the impact of LE-127/2 on the expression of basic proteins (LC-3, Beclin-1 and p62) involved in the process of autophagy in the three different melanoma cell lines studied. Elevated expression of these proteins was detected as a result of LE-127/2 (20 ?M) treatment. LE-127/2 also induced the expression of some proteins involved in apoptosis, and it is particularly noteworthy the increased level of cleaved PARP. Based on the results obtained, it can be concluded that LE-127/2 induced autophagy could lead to the inhibition of cell proliferation and death in melanoma cells.
Tárgyszavak:	Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Melanoma CBG LE-127/2 Vemurafenib Autophagy Apoptosis
Megjelenés:	European Journal Of Pharmaceutical Sciences. - 203 (2024), p. 1-15. -
További szerzők:	Szabó Zsuzsanna (1973-) (biológus, biológia-kémia szakos középiskolai tanár) Király József (1995-) (gyógyszer-biotechnológus) Lőrincz Eszter Boglárka (1993-) (vegyész) Vass Virág (1986-) (biológus) Tánczos Bence (1987-) (biológus) Bereczki Ilona (1981-) (vegyész, antibiotikumkémikus) Herczegh Pál (1947-) (vegyész, antibiotikumkémikus) Remenyik Éva (1956-) (bőrgyógyász) Tósaki Árpád (1958-) (kísérletes farmakológus, gyógyszerész) Szabó Erzsébet (1973-) (molekuláris biológus)
Pályázati támogatás:	K-124719 OTKA FK 142315 OTKA
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001-es BibID:	BIBFORM110822
035-os BibID:	(Scopus)85152907635
Első szerző:	Vass Virág (biológus)
Cím:	Reperfusion-induced injury and the effects of the dithioacetate type hydrogen sulfide donor ibuprofen derivative, BM-88, in isolated rat hearts / Virág Vass, Erzsébet Szabó, Ilona Bereczki, Nóra Debreczeni, Anikó Borbás, Pál Herczegh, Árpád Tósaki
Dátum:	2023
ISSN:	0928-0987
Tárgyszavak:	Orvostudományok Gyógyszerészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk
Megjelenés:	European Journal Of Pharmaceutical Sciences. - 185 (2023), p. 1-14. -
További szerzők:	Szabó Erzsébet (1973-) (molekuláris biológus) Bereczki Ilona (1981-) (vegyész, antibiotikumkémikus) Debreczeni Nóra (1993-) (vegyész) Borbás Anikó (1965-) (vegyész) Herczegh Pál (1947-) (vegyész, antibiotikumkémikus) Tósaki Árpád (1958-) (kísérletes farmakológus, gyógyszerész)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00043 GINOP NKFIH-K-124719 Egyéb
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