Találati lista | Tudóstér

001-es BibID:	BIBFORM097487
Első szerző:	Kenesei Ádám
Cím:	IL-15 Trans-Presentation Is an Autonomous, Antigen-Independent Process / Kenesei Ádám, Volkó Julianna, Szalóki Nikoletta, Mocsár Gábor, Jambrovics Károly, Balajthy Zoltán, Bodnár Andrea, Tóth Katalin, Waldmann Thomas A., Vámosi György
Dátum:	2021
ISSN:	0022-1767 1550-6606
Megjegyzések:	IL-15 plays a pivotal role in the long-term survival of T cells and immunological memory. Its receptor consists of three subunits (IL-15R?, IL-2/15R?, and ?c). IL-15 functions mainly via trans-presentation (TP), during which an APC expressing IL-15 bound to IL-15R? presents the ligand to the ??c receptor-heterodimer on a neighboring T/NK cell. To date, no direct biophysical evidence for the intercellular assembly of the IL-15R heterotrimer exists. Ag presentation (AP), the initial step of T cell activation, is also based on APC?T cell interaction. We were compelled to ask whether AP has any effect on IL-15 TP or whether they are independent processes. In our human Raji B cell?Jurkat T cell model system, we monitored inter-/intracellular protein interactions upon formation of IL-15 TP and AP receptor complexes by Förster resonance energy transfer measurements. We detected enrichment of IL-15R? and IL-2/15R? at the synapse and positive Förster resonance energy transfer efficiency if Raji cells were pretreated with IL-15, giving direct biophysical evidence for IL-15 TP. IL-15R? and MHC class II interacted and translocated jointly to the immunological synapse when either ligand was present, whereas IL-2/15R? and CD3 moved independently of each other. IL-15 TP initiated STAT5 phosphorylation in Jurkat cells, which was not further enhanced by AP. Conversely, IL-15 treatment slightly attenuated Ag-induced phosphorylation of the CD3? chain. Our studies prove that in our model system, IL-15 TP and AP can occur independently, and although AP enhances IL-15R assembly, it has no significant effect on IL-15 signaling during TP. Thus, IL-15 TP can be considered an autonomous, Ag-independent process.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk interleukin trans-presentation immunology
Megjelenés:	Journal Of Immunology. - 207 : 10 (2021), p. 2489-2500. -
További szerzők:	Volkó Julianna (1983-) (biotechnológus) Szalóki Nikoletta (1981-) (biológus) Mocsár Gábor (1981-) (biofizikus) Jambrovics Károly (1988-) (biológus, gyógyszer-biotechnológus) Balajthy Zoltán (1957-) (biokémikus, sejtbiológus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Tóth Katalin Ágnes (1977-) (biokémikus, molekuláris biológus) Waldmann, Thomas A. Vámosi György (1967-) (biofizikus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00026 GINOP EFOP-3.6.3-VEKOP-16-2017-00009 EFOP Tempus Public Foundation 273478 Egyéb Deutscher Akademischer Austauschdienst 273478 Egyéb HHS \| NIH \| National Cancer Institute intramural research program Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Saját polcon:

001-es BibID:	BIBFORM004690
035-os BibID:	(scopus)0035191769 (wos)000172278000004
Első szerző:	Pfeiffer, Alexandra
Cím:	Lipopolysaccharide and ceramide docking to CD14 provokes ligand-specific receptor clustering in rafts / Pfeiffer, A., Bottcher, A., Orso, E., Kapinsky, M., Nagy, P., Bodnar, A., Spreitzer, I., Liebisch, G., Drobnik, W., Gempel, K., Horn, M., Holmer, S., Hartung, T., Multhoff, G., Schutz, G., Schindler, H., Ulmer, A. J., Heine, H., Stelter, F., Schutt, C., Rothe, G., Szollosi, J., Damjanovich, S., Schmitz, G.
Dátum:	2001
Megjegyzések:	The glycosylphosphatidylinositol-anchored receptor CD14 plays a major role in the inflammatory response of monocytes to lipopolysaccharide. Here, we describe that ceramide, a constituent of atherogenic lipoproteins, binds to CD14 and induces clustering of CD14 to co-receptors in rafts. In resting cells, CD14 was associated with CD55, the Fcgamma-receptors CD32 and CD64 and the pentaspan CD47. Ceramide further recruited the complement receptor 3 (CD11b/CD18) and CD36 into proximity of CD14. Lipopolysaccharide, in addition, induced co-clustering with Toll-like receptor 4, Fcgamma-RIIIa (CD16a) and the tetraspanin CD81 while CD47 was dissociated. The different receptor complexes may be linked to ligand-specific cellular responses initiated by CD14.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Antigens,CD Antigens,CD14 Carrier Proteins Ceramides chemistry Glycoproteins Human Inflammation Ligands Lipopolysaccharides Macrophage-1 Antigen Membrane Glycoproteins Membrane Microdomains metabolism Monocytes pharmacology Receptors,Cell Surface Support,Non-U.S.Gov't
Megjelenés:	European Journal of Immunology. - 31 : 11 (2001), p. 3153-3164. -
További szerzők:	Böttcher, Alfred Orsó Evelyn Kapinsky, Michael Nagy Péter (1971-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Spreitzer, Ingo Liebisch, Gerhard Drobnik, Wolfgang Gempel, Klaus Horn, Markus Holmer, Stefan Hartung, Thomas Multhoff, Gabriele Schütz, Gerhard Schindler, Hansgeorg Ulmer, Artur J. Heine, Holger Stelter, Felix Schütt, Christine Rothe, Gregor Szöllősi János (1953-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Schmitz, Gerd
Internet cím:	DOI
Borító:
Saját polcon: