CCL

Összesen 41 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM046289
Első szerző:Bacsó Zsolt (biofizikus)
Cím:A photobleaching energy transfer analysis of CD8MHC-I and LFA-1ICAM-1 interactions in CTL-target cell conjugates / Bacsó Zsolt, Bene László, Bodnár Andrea, Matkó János, Damjanovich Sándor
Dátum:1996
ISSN:0165-2478
Megjegyzések:The photobleaching energy transfer (pbFRET) technique is a fluorescence method to measure proximity relationships between molecules, especially cell surface proteins, labeled with fluorophore-conjugated monoclonal antibodies, on a pixel-by-pixel base using digital imaging microscopy. This technique enables analysis of inter- and intramolecular proximities at cell surfaces at physiological conditions. We have developed a pbFRET approach to measure intercellular proximities in order to access spatial organization of interacting proteins in the contact region of two 'communicating' cells. Two examples, as possible application areas of this approach, are presented here: interaction between CD8 and MHC-I molecules in point contacts and interaction between LFA-1 and ICAM-1 molecules in focal contacts of CTL-target conjugates. The geometry of these protein contacts based on our resonance energy transfer (RET) data is consistent with the observed blocking effects of monoclonal antibodies (directed against the interacting proteins) on the cytolytic activity of CTLs and suggest a critical role for CD8beta-subunit in signal transmission in peripheral T-lymphocytes
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Immunology Letters. - 54 : 2-3 (1996), p. 151-156. -
További szerzők:Bene László (1963-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Matkó János (1952-) (biológus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM035548
Első szerző:Bacsó Zsolt (biofizikus)
Cím:Intercellular photobleaching energy transfer measurement monitoring recognition processes / Bacso, Z., Bene, L., Matko, J., Damjanovich, S.
Dátum:1996
ISSN:0079-6107
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
egyetemen (Magyarországon) készült közlemény
Megjelenés:Progress In Biophysics & Molecular Biology. - 65 : 1 (1996), p. PC122. -
További szerzők:Bene László (1963-) (biofizikus) Matkó János (1952-) (biológus) Damjanovich Sándor (1936-2017) (biofizikus)
Borító:

3.

001-es BibID:BIBFORM004699
Első szerző:Bacsó Zsolt (biofizikus)
Cím:INF-gamma rearranges membrane topography of MHC-I and ICAM-1 in colon carcinoma cells / Bacso, Z., Bene, L., Damjanovich, L., Damjanovich, S.
Dátum:2002
Megjegyzések:Flow-cytometric fluorescence energy transfer (FCET) measurements between fluorescently labeled cell surface MHC-I and ICAM-1 molecules indicated similar receptor patterns in the plasma membrane of interferon-gamma (INF-gamma)-treated colon carcinoma cells as those observed earlier at the surface of lymphoid cells. INF-gamma activation significantly increased the density of MHC-I and ICAM-1 proteins in the membrane. This increase in receptor density was accompanied by decreased proximity level of the homo-associated MHC-I receptors. Hetero-association of MHC-I and ICAM-1 molecules was increased by INF-gamma treatment. INF-gamma changed neither hetero- nor homo-association of transferrin receptors. By staining the sphingomyelin/cholesterol-enriched lipid microdomains with fluorescently labeled cholera toxin B subunit, we found an increase in the amount of lipid-raft associated G(M1)-gangliosides due to INF-gamma treatment. Confocal microscopic results and FCET measurements show that MHC-I and ICAM-1 are components of G(M1)-ganglioside containing lipid-rafts and also support an increase in the size of these lipid-rafts upon INF-gamma treatment.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Carcinoma
Cell Membrane
chemistry
Cholera Toxin
Colonic Neoplasms
drug effects
Energy Transfer
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescence
G(M1) Ganglioside
Histocompatibility Antigens Class I
Human
Hungary
Intercellular Adhesion Molecule-1
Interferon Type II
Membrane Microdomains
metabolism
Microscopy,Confocal
pathology
pharmacology
Protein Binding
Receptors,Cell Surface
Receptors,Transferrin
Support,Non-U.S.Gov't
Tumor Cells,Cultured
Megjelenés:Biochemical and Biophysical Research Communications. - 290 : 2 (2002), p. 635-640. -
További szerzők:Bene László (1963-) (biofizikus) Damjanovich László (1960-) (általános sebész) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:DOI
elektronikus változat
Borító:

4.

001-es BibID:BIBFORM059572
Első szerző:Bene László (biofizikus)
Cím:Nanoparticle energy transfer on the cell surface / László Bene, Gergely Szentesi, László Mátyus, Rezső Gáspár, Sándor Damjanovich
Dátum:2005
ISSN:0952-3499
Megjegyzések:Membrane topology of receptors plays an important role in shaping transmembrane signalling of cells. Among the methods used for characterizing receptor clusters, fluorescence resonance energy transfer between a donor and acceptor fluorophore plays a unique role based on its capability of detecting molecular level (2-10 nm) proximities of receptors in physiological conditions. Recent development of biotechnology has made possible the usage of colloidal gold particles in a large size range for specific labelling of cells for the purposes of electron microscopy. However, by combining metal and fluorophore labelling of cells, the versatility of metal-fluorophore interactions opens the way for new applications by detecting the presence of the metal particles by the methods of fluorescence spectroscopy. An outstanding feature of the metal nanoparticle-fluorophore interaction is that the metal particle can enhance spontaneous emission of the fluorophore in a distance-dependent fashion, in an interaction range essentially determined by the size of the nanoparticle. In our work enhanced fluorescence of rhodamine and cyanine dyes was observed in the vicinity of immunogold nanoparticles on the surface of JY cells in a flow cytometer. The dyes and the immunogold were targetted to the cell surface receptors MHCI, MHCII, transferrin receptor and CD45 by monoclonal antibodies. The fluorescence enhancement was sensitive to the wavelength of the exciting light, the size and amount of surface bound gold beads, as well as the fluorophore-nanoparticle distance. The intensity of 90 degrees scattering of the incident light beam was enhanced by the immunogold in a concentration and size-dependent fashion. The 90 degrees light scattering varied with the wavelength of the incident light in a manner characteristic to gold nanoparticles of the applied sizes. A reduction in photobleaching time constant of the cyanine dye was observed in the vicinity of gold particles in a digital imaging microscope. Modulations of 90 degrees light scattering intensity and photobleaching time constant indicate the role of the local field in the fluorescence enhancement. A mathematical simulation based on the electrodynamic theory of fluorescence enhancement showed a consistency between the measured enhancement values, the inter-epitope distances and the quantum yields. The feasibility of realizing proximity sensors operating at distance ranges larger than that of the conventional Forster transfer is demonstrated on the surface of living cells
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antibodies
Antibodies,Monoclonal
Antigens,CD45
B-Lymphocytes
Biophysics
Carbocyanines
Cell Membrane
Cells
Cultured
chemistry
diagnostic use
Dyes
Energy Transfer
Fluorescence
Fluorescent Dyes
Gold
Humans
Hungary
immunology
Light
metabolism
methods
Microscopy
Nanotechnology
Photobleaching
Receptors,Transferrin
Research
Rhodamines
Spectrometry,Fluorescence
Support
Megjelenés:Journal Of Molecular Recognition. - 18 : 3 (2005), p. 236-253. -
További szerzők:Szentesi Gergely (1976-) (kémia-fizika tanár) Mátyus László (1956-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
Pályázati támogatás:OTKA-T042618
OTKA
OTKA-TS040773
OTKA
OTKA-T043509
OTKA
OTKA-T043087
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

5.

001-es BibID:BIBFORM006027
Első szerző:Bene László (biofizikus)
Cím:Lateral organization of the ICAM-1 molecule at the surface of human lymphoblasts : a possible model for its co-distribution with the IL-2 receptor, class I and class II HLA molecules / Bene L., Balázs M., Matkó J., Möst J., Dierich M. P., Szöllösi J., Damjanovich S.
Dátum:1994
Megjegyzések:Lateral distribution of the ICAM-1 molecule and its topological relationship (mutual proximity) to the heavy and light chains of class I HLA molecules, HLA-DR and interleukin-2 receptor alpha-chain (IL-2R alpha) were studied in the plasma membrane of HUT-102B2 T and JY B lymphoblastoid cell lines by the technique of flow cytometric energy transfer (FCET). Effects of adherency and treatments with recombinant interferon-gamma or tumor necrosis factor-alpha on the relative expression level of ICAM-1 to the above cell surface proteins were also investigated. While the cytokines did not significantly affect the ICAM-1 level of either cell line, an increased ICAM-1 expression was found on adherent JY cells. The ICAM-1 expression varied significantly with the cell cycle and culture conditions, as well. The statistical analysis of the differences observed in the energy transfer efficiency histograms resulted in a possible model of lateral co-distribution of these proteins in the plasma membrane. These two-dimensional patterns proved to be different for T and B lymphoma lines. ICAM-1 molecules showed a high degree of self-association on HUT-102B2 (T) cells, while they were mainly expressed as monomers on the surface of JY (B) cells. Both cells showed a significant (ca. 30%) difference between densities of the heavy and light chains of class I HLA antigen, suggesting a substantial amount of beta 2-microglobulin free heavy chains on these cell lines. The class I HLA molecules also showed partial self-association, but on both cell lines. The beta 2-microglobulin and the heavy chain of the class I HLA showed strongly different proximities to the IL-2R alpha, HLA-DR and ICAM-1 molecules, indicating that their orientations relative to the other proteins are dissimilar. IL-2R alpha molecules of the HUT-102B2 (T) cells are located mostly in the vicinity of the beta 2-microglobulin. In contrast, the local density of HLA-DR antigens is higher in the proximity of the heavy chain than in the vicinity of the beta 2-microglobulin. The possible functional significance of these protein patterns is also discussed herein.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
analysis
Antibodies,Monoclonal
B-Lymphocyte Subsets
beta 2-Microglobulin
Cell Adhesion
Cell Adhesion Molecules
Cell Cycle
Cell Line
Energy Transfer
Flow Cytometry
Histocompatibility Antigens Class I
HLA Antigens
HLA-D Antigens
HLA-DR Antigens
Human
Hungary
immunology
Intercellular Adhesion Molecule-1
Interferon Type II
Interleukin-2
Light
physiology
Receptors,Interleukin-2
Support,Non-U.S.Gov't
Support,U.S.Gov't,Non-P.H.S.
T-Lymphocyte Subsets
Tumor Necrosis Factor
Megjelenés:European Journal of Immunology. - 24 : 9 (1994), p. 2115-2123. -
További szerzők:Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Matkó János (1952-) (biológus) Most, J. Dierich, Manfred P. Szöllősi János (1953-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:DOI
Borító:

6.

001-es BibID:BIBFORM004858
Első szerző:Bene László (biofizikus)
Cím:Detection of receptor trimers on the cell surface by flow cytometric fluorescence energy homotransfer measurements / László Bene, János Szöllősi, Gergely Szentesi, László Damjanovich, Rezső Gaspar, Thomas A. Waldmann, Sándor Damjanovich
Dátum:2005
ISSN:0006-3002
Megjegyzések:Fluorescence energy homotransfer offers a powerful tool for the investigation of the state of oligomerization of cell surface receptors on a cell-by-cell basis by measuring the polarized components of fluorescence intensity of cells labeled with fluorescently stained antibodies. Here we describe homotransfer-based methods for the flow cytometric detection and analysis of hetero- and homo-associations of cell surface receptors. Homotransfer efficiencies for two- and three-body energy transfer interactions are defined and their frequency distribution curves are computed from the fluorescence anisotropy distributions of multiple-labeled cells. The fractions of receptors involved in homo-clustering is calculated based on the dependence of the fluorescence anisotropy on the surface concentration of the fluorescently stained antibodies. A homotransfer analysis of the homo- and hetero-clustering of the MHCI and MHCII glycoproteins, the cytokine receptor IL-2Ralpha, transferrin receptor and the receptor-type tyrosine phosphatase CD45 on JY B and Kit-225-K6 T cells is presented. We investigated how various factors such as the type of dye, rotational mobility of the dye and dye-targeting antibody, as well as the wavelength of the exciting light affect the homotransfer. We show that the homotransfer technique combined with the high statistical resolution of flow cytometry is an effective tool for detecting different oligomeric states of receptors by using fluorophores having restricted rotational mobility on the time scale of fluorescence
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Fluorescence anisotropy
Receptor clustering
MHCI and MHCII glycoprotein
IL-2Rα
Transferrin receptor
CD45
Megjelenés:Biochimica et Biophysica Acta (BBA). Molecular Cell Research. - 1744 : 2 (2005), p. 176-198. -
További szerzők:Szöllősi János (1953-) (biofizikus) Szentesi Gergely (1976-) (kémia-fizika tanár) Damjanovich László (1960-) (általános sebész) Gáspár Rezső (1944-) (biofizikus) Waldmann, Thomas A. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
DOI
Borító:

7.

001-es BibID:BIBFORM004939
Első szerző:Bene László (biofizikus)
Cím:Major histocompatibility complex class I protein conformation altered by transmembrane potential changes / Bene, L., Szollosi, J., Balazs, M., Matyus, L., Gaspar, R., Ameloot, M., Dale, R. E., Damjanovich, S.
Dátum:1997
ISSN:0196-4763
Megjegyzések:The nature of charge distributions in membrane-bound macromolecular structures renders them susceptible to interaction with transmembrane potential fields. As a result, conformational changes in such species may be expected to occur when this potential is altered. We have detected reversible conformational change in the major histocompatibility complex (MHC) class I antigen in the plasma membrane of human JY cells, as monitored by flow-cytometric resonance energy-transfer, upon reduction of the transmembrane potential (depolarization). This change increased the intramolecular energy-transfer efficiency between fluorescent donor- and acceptor-labeled monoclonal antibodies directed, respectively, to epitopes on the light (beta 2-microglobulin) and the heavy chains of the MHC class I antigen. Repolarization of the depolarized samples restored the energy-transfer efficiency to the original values measured before depolarization. Depolarization caused similar relative changes in fluorescence resonance energy-transfer efficiency when Fab fragments were used for labeling MHC class I complex, suggesting that the observed phenomenon is not restricted to whole monoclonal antibodies.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antibodies, Monoclonal
Antigen Presentation
B-Lymphocytes
beta 2-Microglobulin
Cell Membrane
chemistry
cytology
Dyes
Energy Transfer
Enzyme Activation
Flow Cytometry
Fluorescein-5-isothiocyanate
Fluorescence
Fluorescent Dyes
Histocompatibility Antigens Class I
Human
Hungary
immunology
Light
Major Histocompatibility Complex
Membrane Potentials
metabolism
methods
Na(+)-K(+)-Exchanging ATPase
Patch-Clamp Techniques
physiology
Protein Conformation
Rhodamines
Surface Properties
Megjelenés:Cytometry. - 27 : 4 (1997), p. 353-357. -
További szerzők:Szöllősi János (1953-) (biofizikus) Balázs Margit (1952-) (sejtbiológus, molekuláris genetikus) Mátyus László (1956-) (biofizikus) Gáspár Rezső (1944-) (biofizikus) Ameloot, Marcel Dale, Robert E. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
Borító:

8.

001-es BibID:BIBFORM004830
035-os BibID:(Scopus)6044239560 (WoS)000223675800046
Első szerző:Bene László (biofizikus)
Cím:Membrane topography of HLA I, HLA II, and ICAM-1 is affected by IFN-gamma in lipid rafts of uveal melanomas / László Bene, Andrea Bodnár, Sándor Damjanovich, György Vámosi, Zsolt Bacsó, János Aradi, András Berta, Judit Damjanovich
Dátum:2004
Megjegyzések:The lateral distribution and colocalization of HLA I, HLA-DR, and ICAM-1 proteins was studied for the first time in the plasma membrane of two human uveal melanoma cell lines, OCM-1 and OCM-3. Our fluorescence resonance energy transfer and confocal laser scanning microscopic experiments revealed that these molecules are mostly confined to the same membrane regions, where they form similar protein patterns (homo- and hetero-associates) to those found previously on other cell types of lymphoid as well as colorectal carcinoma origin. Confocal microscopic colocalization experiments with GM(1) gangliosides and the GPI-anchored CD59 molecules showed enrichment of HLA I, HLA-DR, and ICAM-1 molecules in specific membrane domains (lipid rafts) excluding the transferrin receptor. IFN-gamma remarkably increased the expression levels of these molecules and rearranged their association patterns, which can affect the adoptive immune response of effector cells
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Antigens
Biophysics
Carcinoma
Cell Line
Cells
Energy Transfer
Flow Cytometry
Fluorescence
Fluorescence Resonance Energy Transfer
Histocompatibility Antigens
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Human
Humans
Hungary
Intercellular Adhesion Molecule-1
Interferon Type II
Melanoma
Membrane Microdomains
metabolism
Microscopy,Confocal
Proteins
Research
Support
Uveal Neoplasms
egyetemen (Magyarországon) készült közlemény
Megjelenés:Biochemical and Biophysical Research Communications. - 322 : 2 (2004), p. 678-683. -
További szerzők:Dóczy-Bodnár Andrea (1970-) (biofizikus) Damjanovich Sándor (1936-2017) (biofizikus) Vámosi György (1967-) (biofizikus) Bacsó Zsolt (1963-) (biofizikus) Aradi János (1942-) (biokémikus, vegyész) Berta András (1955-) (szemész, gyermekszemész) Damjanovich Judit (1963-) (szemész)
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

9.

001-es BibID:BIBFORM004657
Első szerző:Bene László (biofizikus)
Cím:Detection of receptor clustering by flow cytometric fluorescence anisotropy measurements / Bene, L., Fulwyler, M. J., Damjanovich, S.
Dátum:2000
Megjegyzések:Perrin equation suggests an alternative way for the accurate energy transfer determination on a cell-by-cell basis by measuring polarized donor intensities in a conventional flow cytometer. METHODS: The relationship between energy transfer and fluorescence anisotropy of the donor was investigated by flow cytometric generation of Perrin-lifetime plots of fluorescent antibody-labeled MHC class I and class II molecules on the surface of living cells. The energy transfer reduced the fluorescence lifetime of the donor. RESULTS: Perrin plots have proven to be sensitive to the segmental mobility of the labeling dye and that of antibodies of different isotypes, and homo-transfer due to the multiple labeling of antibodies. A method demonstrating the feasibility of energy transfer determination by measuring anisotropy enhancement of the donor is presented. Flow cytometric histograms of the donor anisotropy and of the deduced energy transfer efficiency are shown, indicating clustering of MHC class I and class II molecules on the surface of human T lymphoblasts. In the Appendix, a method for the simultaneous determination of both energy transfer efficiency and donor fluorescence anisotropy, without need for G-factor measurement, is also presented. CONCLUSIONS: We demonstrate that energy transfer efficiency, i.e., proximity, between suitably selected donor and acceptor, and the rotational relaxation of the donor, i.e., donor mobility, can be simultaneously measured in a flow cytometer
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Adult
analysis
Antibodies
beta 2-Microglobulin
Cells
diagnostic use
Dyes
Energy Transfer
Flow Cytometry
Fluorescence
Fluorescence Polarization
Fluorescent Dyes
Histocompatibility Antigens Class I
Histocompatibility Antigens Class II
Human
Hungary
Immunoglobulin G
immunology
methods
Receptors,Cell Surface
Support,Non-U.S.Gov't
T-Lymphocytes
Megjelenés:Cytometry. - 40 : 4 (2000), p. 292-306. -
További szerzők:Fulwyler, Mack J. Damjanovich Sándor (1936-2017) (biofizikus)
Internet cím:elektronikus változat
DOI
Borító:

10.

001-es BibID:BIBFORM046087
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Signal transduction in T lymphocytes and aging / Damjanovich S., Gaspar R., Bene L., Jenei A., Matyus L.
Dátum:2003
ISSN:0531-5565
Megjegyzések:Subclasses of cells in different compartments of the immune system possesses all those attributes, that make them suitable though somewhat limited models for the investigation of cellular processes during aging. Blood samples provide relative easily high amount of cells belonging to the same subclass, all of them having complex cascade processes in their signal transduction mechanisms, therefore being excellent targets for such investigations. One such subclass comprises peripheral blood lymphocytes. The signal-transduction cascade across the plasma membrane of lymphocytes displays many of the general features enabling us to draw conclusions for other cellular signaling problems that may arise during aging in other cell types not directly related to the immune system. The advantage of this approach lies in the fact that sometimes it is extremely difficult to study signal transduction processes in certain cell types under physiological conditions. The simultaneous occurrence of physical, chemical and molecular biological regulation of the immune processes at cellular and network levels make them very good examples for focusing our interest also on similar processes in other systems and cells. The fast developing new measuring techniques and the rapidly accumulating experimental data make it relatively easy to provide interesting new aspects, and ideas in this field. Finally, the immune system itself has its great importance and after all, it has an obvious declination with aging, the immune-senescence.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Experimental Gerontology. - 38 : 3 (2003), p. 231-236. -
További szerzők:Gáspár Rezső (1944-) (biofizikus) Bene László (1963-) (biofizikus) Jenei Attila (1966-) (biofizikus) Mátyus László (1956-) (biofizikus)
Pályázati támogatás:T029947
OTKA
T030411
OTKA
F034487
OTKA
TS040773
OTKA
T42618
OTKA
T43087
OTKA
T43509
OTKA
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

11.

001-es BibID:BIBFORM038681
035-os BibID:PMID:12489751
Első szerző:Damjanovich Sándor (biofizikus)
Cím:New trends in studying structure and function of biological membranes / S. Damjanovich, G. Vámosi, Andrea Bodnár, L. Bene
Dátum:2002
ISSN:0231-424X
Megjegyzések:Thirty years ago Singer and Nicolson constructed the "fluid mosaic model" of the membrane, which described the structural and functional characteristics of the plasma membrane of non-polarized cells like circulating blood lymphocytes as a fluid lipid phase accommodating proteins with a relatively free mobility. It is a rare phenomenon in biology that such a model could survive 30 years and even today it has a high degree of validity. However, in the light of new data it demands some modifications. In this minireview we present a new concept, which revives the SN model, by shifting the emphasis from fluidity to mosaicism, i.e. to lipid microdomains and rafts. A concise summary of data and key methods is given, proving the existence of non-random co-distribution patterns of different receptor kinds in the microdomain system of the plasma membrane. Furthermore we present evidence that proteins are not only accommodated by the lipid phase, but they are integral structural elements of it. Novel suggestions to the SN model help to develop a modernized version of the old paradigm in the light of new data.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény hazai lapban
Animals
Biophysics
blood
Cell Membrane
Cells
Humans
Light
Lipid Metabolism
Lymphocytes
metabolism
methods
Microscopy, Electron, Scanning
Models, Biological
Proteins
Receptors, Cell Surface
Research
Support
trends
ultrastructur
egyetemen (Magyarországon) készült közlemény
Megjelenés:Acta Physiologica Hungarica. - 89 : 4 (2002), p. 415-425. -
További szerzők:Vámosi György (1967-) (biofizikus) Dóczy-Bodnár Andrea (1970-) (biofizikus) Bene László (1963-) (biofizikus)
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

12.

001-es BibID:BIBFORM035610
Első szerző:Damjanovich Sándor (biofizikus)
Cím:Mobility of HLA Class I antigen is influenced by anti-CD4 monoclonal antibody in lymphocyte membranes. A flow cytometric energy transfer, fluorescence photobleaching recovery and rotational relaxation study / Sandor Damjanovich; Laszlo Balkay; Laszlo Pohubi; Tamas Varhelyi; Laszlo Bene; Adorjan Aszalos; Laszlo Matyus; Lajos Tron
Dátum:1990
Tárgyszavak:Orvostudományok Elméleti orvostudományok előadáskivonat
Megjelenés:Time-Resolved Laser Spectroscopy in Biochemistry II. / szerk. Lakowicz, J. R. - p. 524-531.
További szerzők:Balkay László (1963-) (biofizikus) Pohubi László (1959-) (fizikus) Várhelyi Tamás Bene László (1963-) (biofizikus) Aszalos Adorján Mátyus László (1956-) (biofizikus) Trón Lajos (1941-) (biofizikus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változa
Borító:
Rekordok letöltése1 2 3 4 
Corvina könyvtári katalógus v10.1.21-SNAPSHOT © 2024 Monguz kft. Minden jog fenntartva.