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001-es BibID:	BIBFORM077294
Első szerző:	Szilágyi Melinda (biológus)
Cím:	Mutation in afsR Leads to A-Factor Deficiency in Streptomyces griseus B2682 / Melinda Szilágyi, Éva Márton, Dávid Lukács, Zsuzsanna Birkó, Zoltán Kele, Sándor Biró
Dátum:	2018
ISSN:	1464-1801
Megjegyzések:	A-factor, a γ-butyrolactone autoregulator, in Streptomyces griseus is involved in the regulation of differentiation and antibiotic production. Here we studied the S. griseus B2682-AFN (A-factor negative) bald mutant that harbors a nonsense mutation in the afsR gene encoding a pleiotropic regulator. Our aim was to prove that this mutation is the cause of the A-factor deficiency in AFN. We also studied whether AfsR regulates A-factor production by AfsA, which is supposed to be the only specific key enzyme in A-factor biosynthesis. METHODS: Wild afsR was cloned to the pHJL401 shuttle vector and was transformed to the S. griseus AFN and B2682 strains. During phenotypic characterization, sporulation, antibiotic, protease, A-factor, and AfsA protein production were studied. RESULTS: Transformation of AFN by a wild afsR restored its phenotype including sporulation, antibiotic, extracellular protease, and A-factor production. Introduction of afsR to the B2682 wild-type strain resulted in antibiotic and extracellular protease overproduction that was accompanied with an elevated A-factor level. AfsA was detected both in AFN and B2682. CONCLUSIONS: AfsR has an effect on the regulation of A-factor production in S. griseus. The presence of AfsA is not sufficient for normal A-factor production. AfsR regulates A-factor biosynthesis independently of AfsA.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:	Journal Of Molecular Microbiology And Biotechnology. - 28 : 5 (2018), p. 216-224. -
További szerzők:	Márton Éva (1992-) (biológus) Lukács Dávid Hádáné Birkó Zsuzsanna (1971-) (molekuláris genetikus) Kele Zoltán Biró Sándor (1949-) (molekuláris genetikus)
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