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001-es BibID:BIBFORM030998
Első szerző:Gonda Sándor (gyógyszerész)
Cím:Effect of High Relative Humidity on Dried Plantago lanceolata L. Leaves during Long-term Storage : Effects on Chemical Composition, Colour and Microbiological Quality / Sándor Gonda, László Tóth, Gyöngyi Gyémánt, Mihály Braun, Tamás Emri, Gábor Vasas
Megjegyzések:Introduction : Modern phytotherapy and quality assurance requires stability data on bioactive metabolites to identify andminimise decomposing factors during processing and storage. A compound's stability in a complex matrix can be differentfrom the stability of the purified compound.Objective To test the stability of iridoids and acteoside and quantify changes in colour and microbiological quality in acommon herbal tea, dried P. lanceolata leaves during exposure to high?humidity air. To test the contribution of fungi tometabolite decomposition.Methodology ? Dried P. lanceolata leaves were exposed to atmospheres of different relative humidity (75, 45 and 0%) for24 weeks. Changes in aucubin and catalpol concentration were determined by CE?MEKC, and those in acteoside on TLC.Colour and chlorophyll?like pigments were measured by different spectrophotometric methods. The number of fungi wasmonitored; 10 strains were isolated from the plant drug, and their ability to decompose the analytes of interest was tested.Results ? During incubation at 75% relative humidity (RH), aucubin, catalpol and acteoside concentrations decreased by 95.7,97.0 and 70.5%, respectively. Strong shifts were detected in CIELAB parameters a* and b* (browning) as a result ofconversion of chlorophyll to pheophytin. Intensive microbial proliferation was also observed. Changes at 45 or 0% RH weretypically insignificant. Seven of the 10 isolated fungal strains could decompose both iridoids, and five could decomposeacteoside in vitro.Conclusion It was shown that exposure to water results in loss of bioactive molecules of P. lanceolata dried leaves, and thatcolonising fungi are the key contributors to this loss.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Plantago lanceolata L.
iridoid stability
long-term storage
microbiological contamination
Megjelenés:Phytochemical Analysis. - 23 : 1 (2012), p. 88-93. -
További szerzők:Tóth László Gyémánt Gyöngyi (1960-) (vegyész) Braun Mihály (1966-) (vegyész, analitikus) Emri Tamás (1969-) (biológus) Vasas Gábor (1975-) (biológus-vegyész)
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