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001-es BibID:	BIBFORM075465
035-os BibID:	(WoS)000427563000023 (Scopus)85042127210
Első szerző:	Jančaříková, Gita
Cím:	Synthesis of α-L-fucopyranoside-presenting glycoclusters and investigation of their interaction with Photorhabdus asymbiotica lectin (PHL) / Gita Jančaříková, Mihály Herczeg, Eva Fujdiarová, Josef Houser, Katalin E. Kövér, Anikó Borbás, Michaela Wimmerová, Magdolna Csávás
Dátum:	2018
ISSN:	0947-6539 1521-3765
Megjegyzések:	Photorhabdus asymbiotica is gram-negative bioluminescent bacteria that is not only as effective an insect pathogen as other members of the genus, but also a bacterium that causes serious diseases in humans. The recently identified bangle lectin PHL from P. asymbiotica verifiably modulates an immune response of humans and insects, which supports the idea that the lectin might play an important role in the host-pathogen interaction. Dimeric PHL contains up to seven L-fucose specific binding sites per monomer, and in order to target multiple binding sites of PHL, alpha-L-fucoside-containing di-, tri- and tetravalent glycoclusters were synthesized. Methyl gallate and pentaerythritol were chosen as multivalent scaffolds, and the fucoclusters were built from the above-mentioned cores by coupling with different oligoethylene bridges and propargyl ?-L-fucosides using 1,3-dipolar azide-alkyne cycloaddition. The interaction between fucoside derivates and PHL was investigated by several biophysical and biological methods, ITC and SPR measurements, hemagglutination inhibition assay and an investigation of bacterial aggregation properties were carried out. Moreover, details of the interaction between PHL and propargyl alpha-L-fucoside as a monomer unit were revealed using X-ray crystallography. Besides this, the interaction with multivalent compounds was studied by NMR techniques. The newly synthesized multivalent fucoclusters proved to be up to several orders of magnitude better ligands than the natural ligand, L-fucose.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk fucoclusters lectin multivalency Photorhabdus asymbiotica agglutination
Megjelenés:	Chemistry-A European Journal. - 24 : 16 (2018), p. 4055-4068. -
További szerzők:	Herczeg Mihály (1979-) (vegyész, biológia-kémia tanár) Fujdiarová, Eva Houser, Josef (1977-) (vegyész) Kövér Katalin, E. (1956-2023) (vegyész) Borbás Anikó (1965-) (vegyész) Wimmerová, Michaela Csávás Magdolna (1977-) (szénhidrátkémikus, vegyész)
Pályázati támogatás:	NKFIH K119509 NKFIH GINOP-2.3.2-15-2016-00008 GINOP
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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