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001-es BibID:	BIBFORM042216
035-os BibID:	PMID:16102115
Első szerző:	Dale, George L.
Cím:	Quantitation of microparticles released from coated-platelets / G. L. Dale, Gy. Remenyi, P. Friese
Dátum:	2005
ISSN:	1538-7933
Megjegyzések:	Dual agonist stimulation of platelets with thrombin and convulxin results in generation of coated-platelets, a sub-population of cells known formerly as COAT-platelets (collagen and thrombin). Coated-platelets retain several procoagulant proteins on their surface and express phosphatidylserine (PS). In this report, we utilize a new methodology to demonstrate that coated-platelets also release microparticles. Platelets were prelabeled with 2.5 microm Bodipy-maleimide and then stimulated with convulxin plus thrombin. Microparticles, 0.3-0.5 microm in diameter, were observed by fluorescence confocal microscopy. Confocal microscopy was also used to demonstrate that microparticles were positive for glycoprotein IIb/IIIa, glycoprotein Ib, CD9, and PS, but negative for fibrinogen and thrombospondin. Furthermore, microparticles released from Bodipy-labeled platelets were observed by flow cytometry, and activation with convulxin plus thrombin produced 15 +/- 5 microparticles per coated-platelet. In contrast, platelets stimulated with thrombin or convulxin alone produced few microparticles. Phenylarsine oxide and diamide, both of which potentiate the mitochondrial permeability transition pore and coated-platelet production, significantly increased the number of microparticles released per coated-platelet.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban convulxin brinogen microparticle platelet thrombin thrombospondin. külföldön készült közlemény
Megjelenés:	Journal of Thrombosis and Haemostasis. - 3 : 9 (2005), p. 2081-2088. -
További szerzők:	Friese, Paul Reményi Gyula (1969-) (belgyógyász, haematológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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