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001-es BibID:	BIBFORM116204
035-os BibID:	(Scopus)85167596616 (WOS)001046691600001
Első szerző:	Szűcs Diána
Cím:	Effect of Inflammatory Microenvironment on the Regenerative Capacity of Adipose-Derived Mesenchymal Stem Cells / Szűcs Diána, Miklós Vanda, Monostori Tamás, Guba Melinda, Kun-Varga Anikó, Póliska Szilárd, Kis Erika, Bende Balázs, Kemény Lajos, Veréb Zoltán
Dátum:	2023
ISSN:	2073-4409
Megjegyzések:	Adipose-derived mesenchymal stem cells are increasingly being used in regenerative medicine as cell therapy targets, including in the treatment of burns and ulcers. The regenerative potential of AD-MSCs and some of their immunological properties are known from in vitro studies; however, in clinical applications, cells are used in non-ideal conditions and can behave differently in inflammatory environments, affecting the efficacy and outcome of therapy. Our aim was to investigate and map the pathways that the inflammatory microenvironment can induce in these cells. High-throughput gene expression assays were performed on AD-MSCs activated with LPS and TNF?. Analysis of RNA-Seq data showed that control, LPS-treated and TNF?-treated samples exhibited distinct gene expression patterns. LPS treatment increased the expression of 926 genes and decreased the expression of 770 genes involved in cell division, DNA repair, the cell cycle, and several metabolic processes. TNF? treatment increased the expression of 174 genes and decreased the expression of 383 genes, which are related to cell division, the immune response, cell proliferation, and differentiation. We also map the biological pathways by further investigating the most altered genes using the Gene Ontology and KEGG databases. Secreted cytokines, which are important in the immunological response, were also examined at the protein level, and a functional assay was performed to assess wound healing. Activated AD-MSC increased the secretion of IL-6, IL-8 and CXCL-10, and also the closure of wounds. AD-MSCs presented accelerated wound healing under inflammation conditions, suggesting that we could use this cell in clinical application.
Tárgyszavak:	Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk adipose-derived mesenchymal stem cells inflammation lipopolysaccharide regenerative medicine tumor necrosis factor
Megjelenés:	Cells. - 12 : 15 (2023), p. 1-19. -
További szerzők:	Miklós Vanda Monostori Tamás Guba Melinda Kun-Varga Anikó Póliska Szilárd (1978-) (biológus) Kis Erika (Szeged) Bende Balázs Kemény Lajos V. (bőrgyógyász Szeged) Veréb Zoltán (1980-) (immunológus, mikrobiológus, molekuláris biológus)
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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