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001-es BibID:	BIBFORM129501
035-os BibID:	(Scopus)105002759098 (WoS)001469242600001
Első szerző:	Farkas Dóra (biomérnök)
Cím:	Transcriptomic response of apple (Malus x domestica Borkh. cv. Húsvéti rozmaring) shoot explants to in vitro cultivation on media containing thidiazuron or 6-benzylaminopurine riboside / Dóra Farkas, Viktor Ambrus, Anita Király, Neama Abdalla, Judit Dobránszki
Dátum:	2025
ISSN:	0167-6857
Megjegyzések:	One of the most critical factors in maintaining healthy in vitro axillary apple shoot cultures is the cytokinin content of the medium. The effects of two cytokinins applied in the medium, thidiazuron and 6-benzylaminopurine riboside, each applied in a quantity of 4.5 ?M, were studied on mRNA transcription of in vitro apple shoots. Transcriptomic response of in vitro apple shoots was investigated at two time points; at 48 h and 4 weeks (at the end of the subculture) after the cytokinin application by mRNA-seq, bioinformatics analysis, GO annotation and KEGG mapping. This study revealed that different expression intensities of DEGs were related to TF families (mainly WRKY, MYB, AP2/ERF, bHLH) associated with plant growth and development, in addition to enzymes involved in genetic and environmental information processing, cell motility, the zeatin biosynthesis, the tryptophan metabolism, the phenylpropanoid biosynthesis, and cutin, suberin,and wax biosynthesis. A putative roadmap based on changes in gene expression intensity that may be in association with various in vitro apple shoot development and growth disorders caused by different cytokinin supplies was conceived.
Tárgyszavak:	Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Plant hormone signal transduction Zeatin biosynthesis DNA replication Transcription factors Malus x domestica Borkh.
Megjelenés:	Plant Cell Tissue and Organ Culture. - 161 : 2 (2025), p. 1-15. -
További szerzők:	Ambrus Viktor Attila (1989-) (biotechnológus) Király Anita (1988-) (biológus) Abdalla, Neama Dobránszki Judit (1967-) (biológus)
Pályázati támogatás:	K146653 OTKA
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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