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001-es BibID:BIBFORM012807
Első szerző:Shemirani, Amir-Houshang (kutató orvos, laboratórium szakorvos)
Cím:Rapid detection of the factor XIII Val34Leu (163 G-->T) polymorphism by real-time PCR using fluorescence resonance energy transfer detection and melting curve analysis / Shemirani A. H., Muszbek L.
Megjegyzések:The Val34Leu polymorphism in the A subunit of blood coagulation factor XIII (FXIII-A) is located in the acti- vation peptide, just three amino acids upstream of the thrombin cleavage site. The Val?Leu replacement accelerates the rate of the proteolytic activation of FXIII and it seems to provide protection against myo- cardial infarction. Methods available for the assess- ment of the FXIII-A Val34Leu polymorphismare rather time-consuming, laborious and not easily applicable for large-scale studies. In this study a new method based on real-time PCR with fluorescence resonance energy transfer (FRET) detection and melting curve analysis was developed. The rapid, simple method was adapted to the widely used real-time PCR instru- ment, LightCycler (Roche Diagnostics). The results showed 100% coincidence with those obtained by the traditional PCR-restriction fragment length polymor- phism (RFLP) assay and fluorescent DNA sequencing. Using this method, an allele frequency of 24.2% was obtained (ns113), which well agrees with the allele frequency obtained by PCR-RFLP on a different group of the same ethnic Hungarian population (25.9%).
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
factor XIII
fluorescence resonance energy transfer
gene polymorphism
real-time PCR
Megjelenés:Clinical Chemistry And Laboratory Medicine. - 42 : 8 (2004), p. 877-879. -
További szerzők:Muszbek László (1942-) (haematológus, kutató orvos)
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