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001-es BibID:	BIBFORM039004
035-os BibID:	PMID:19851727
Első szerző:	Penyige András (molekuláris genetikus)
Cím:	Analysis and identification of ADP-ribosylated proteins of Streptomyces coelicolor M145 / András Penyige, Judit Keseru, Ferenc Fazakas, Iván Schmelczer, Krisztina Szirák, György Barabás, Sándor Biró
Dátum:	2009
ISSN:	1225-8873
Megjegyzések:	Mono-ADP-ribosylation is the enzymatic transfer of ADP-ribose from NAD(+) to acceptor proteins catalyzed by ADP-ribosyltransferases. Using m-aminophenylboronate affinity chromatography, 2D-gel electrophoresis, in-gel digestion and MALDI-TOF analysis we have identified eight in vitro ADP-ribosylated proteins in Streptomyces coelicolor, which can be classified into three categories: (i) secreted proteins; (ii) metabolic enzymes using NAD(+)/NADH or NADP(+)/NADPH as coenzymes; and (iii) other proteins. The secreted proteins could be classified into two functional categories: SCO2008 and SC05477 encode members of the family of periplasmic extracellular solute-binding proteins, and SCO6108 and SC01968 are secreted hydrolases. Dehydrogenases are encoded by SC04824 and SC04771. The other targets are GlnA (glutamine synthetase I., SC02198) and SpaA (starvation-sensing protein encoded by SC07629). SCO2008 protein and GlnA had been identified as ADP-ribosylated proteins in previous studies. With these results we provided experimental support for a previous suggestion that ADP-ribosylation may regulate membrane transport and localization of periplasmic proteins. Since ADP-ribosylation results in inactivation of the target protein, ADP-ribosylation of dehydrogenases might modulate crucial primary metabolic pathways in Streptomyces. Several of the proteins identified here could provide a strong connection between protein ADP-ribosylation and the regulation of morphological differentiation in S. coelicolor.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban protein ADP-ribosylation Streptomyces coelicolor MALDI-TOF 2-D PAGE egyetemen (Magyarországon) készült közlemény
Megjelenés:	Journal Of Microbiology. - 47 : 5 (2009), p. 549-556. -
További szerzők:	Fazakas Ferenc (1969-) (molekuláris biológus) Schmelczer Iván Keserű Judit (1976-) (molekuláris genetikus) Szirák Krisztina (1973-) (molekuláris genetikus) Barabás György (1933-) (sejtbiológus, molekuláris genetikus) Biró Sándor (1949-) (molekuláris genetikus)
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