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001-es BibID:BIBFORM061576
Első szerző:Jakab Attila (szülész-nőgyógyász, endokrinológus)
Cím:Correlation between sperm chromatin decondensation, density, aneuploidy and hyaluronic binding capacity / Jakab, A., Benyó, M., Mokanszki, A., Bazsáné, K. Z., Molnár, Z.
Megjegyzések:Study question: Whether there is relation between sperm chromatin deconden- sation, concentration, hialuronic acid (HA) binding capacity and aneuploidy of semen samples of fertile, normozoospermic and infertile, oligozoospermic men?Summary answer: There are correlations between sperm condensation and the examined biomarkers, irrespective to fertility status. The sperm conden- sation rate, HA bindig capacity related fertility potential and sperm density are strongly related. Also, there is relation between sperm condensation and aneuploidy rate.What is known already: Importance of sperm biomarkers are increasing in male infertility. Beside genetic integrity and fertilization potential tests, epigen- etic studies came to the front. Chromatin decondensation is related to persistent histones detected by aniline blue, while HA binding capacity reflects genetic integrity and maturity related fertilization potential. Paralel evaluation of test results improves the understanding the regulation mechanisms of spermatogen- esis and fertilization, may help in choosing the most effective therapeutic ap- proach and sperm selection for assisted reproduction.Study design, size, duration: This is a cross-sectional study examining semen of 20 infertile, oligozoospermic and 20 fertile, normozoospermic men attend- ing at the Andrology Center and the Sperm Cryopreservation Laboratory. Participants/materials, setting, methods: The ejaculates were assessed as per WHO guidelines. Hyaluronic acid binding capacity test was the func- tional test. To determine the numerical chromosome aberrations we used multicolor fluorescent in situ hybridization (FISH) for chromosomes X, Y and 17. Aniline blue staining was performed as a nuclear decondensation marker test.Main results and the role of chance: In normozoospermic samples mean sperm concentration was 73,4 M/mL, rate of decondensed sperm nuclei detected by aniline blue 16.2%, HA-binding capacity 82% and estimated an- euploidy rate 4.12%. In the oligozoospermic samples mean sperm concentra- tion was 7.13 M/mL, rate of dencondensed nuclei 41.8%, HA binding capacity 47.5% and the estimated aneuploidy rate 6.17%. All examined parameters dif- fer significantly, if samples below and over 20% decondensation compared. There are inverse correlations between sperm decondensation and sperm con- centration (r = -0.876), as well as sperm decondensation and hyaluronic binding capacity (r = -0.876). Sperm decondensation rate is also related to aneuploidy rate (r = 0.559).Limitations, reason for caution: Data on confounders influencing sperm characteristics such as smoking, occupational or environmental hazards, co- morbidities were not collected.Wider implications of the findings: Although chromatin condensation occurs in late phase of spermatogenesis, it shows correlation with cytogenetic abnor- malities and membrane remodelling, irrespective to the fertility status. It can be explained with a common regulation mechanism, thus malfunction of it can result in various sperm defects on different levels. However, since examined biomarkers reflect different sperm defects, they cannot replace each other. Study funding/competing interest(s): Funding by national/international organization(s) ? This research was supported by the European Union and the State of Hungary, co-financed by the European Social Fund in the framework of TÁMOP 4.2.4.A/ 2-11/1-2012-0001Trial registration number: NA.Keywords: sperm function, sperm biomarkers, sperm decondensation, sperm aneuploidy, hyaluronic binding capacity
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
sperm function
sperm biomarkers
sperm decondensation
sperm aneuploidy
sperm hyaluronic bindig capacity
Megjelenés:Human Reproduction. - 30 : Suppl (2015). p. i159. -
További szerzők:Benyó Mátyás (1982-) (urológus) Mokánszki Attila (1983-) (molekuláris biológus Ph.D hallgató) Bazsáné Kassai Zsuzsa (1946-) (embriológus) Molnár Zsuzsanna (1980-) (szülész-nőgyógyász, labor szakorvosjelölt, urológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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