Összesen 1 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM068608
035-os BibID:(cikkazonosító)9795271 (WOS)000402835500001 (Scopus)85021663462
Első szerző:Hudák Renáta
Cím:The phosphatase inhibitor calyculin-A impairs clot retraction, platelet activation and thrombin generation / Hudák Renáta, Vincze János, Csernoch László, Bekéné Debreceni Ildikó, Oláh Tamás, Erdődi Ferenc, Kenneth J. Clemetson, Kappelmayer János
Dátum:2017
ISSN:2314-6133 2314-6141
Megjegyzések:The aim of this study was to investigate the effect of the serine/threonine protein phosphatase inhibitor, calyculin-A (CLA) on clot formation and on the procoagulant activity of human platelets. Platelet rich plasma (PRP) samples were preincubated with buffer or CLA and subsequently platelets were activated by the protease-activated receptor 1 (PAR-1) activator, thrombin-receptor activating peptide (TRAP). Clot retraction was detected by observing clot morphology up to 1 hour, phosphatidylserine (PS)-expression was studied by flow cytometry and thrombin generation was measured by a fluorimetric assay. For the intracellular Ca2+ assay, platelets were loaded with calcium-indicator dyes and the measurements were carried out using a ratiometric method with real-time confocal microscopy. CLA preincubation inhibited clot retraction, PS-expression and thrombin formation. TRAP activation elicited Ca2+ response and PS-expression in a subset of platelets. The activated PRP displayed significantly faster and enhanced thrombin generation compared to non-activated samples. CLA pretreatment abrogated PS-exposure and clot retraction also in TRAP-activated samples. As a consequence of the inhibitory effect on calcium elevation and PS-expression, CLA significantly downregulated thrombin generation in PRP. Our results show that CLA-pretreatment may be a useful tool to investigate platelet activation mechanisms that contribute to clot formation and thrombin generation.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
phosphatases
calyculin-A
phosphatidylserine
thrombin generation
Megjelenés:Biomed Research International. - 2017 (2017), p. 1-10. -
További szerzők:Vincze János (1988-) (orvos) Csernoch László (1961-) (élettanász) Bekéné Debreceni Ildikó Oláh Tamás (1983-) (élettanász) Erdődi Ferenc (1953-) (biokémikus) Clemetson, Kenneth J. Kappelmayer János (1960-) (laboratóriumi szakorvos)
Internet cím:DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1