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001-es BibID:BIBFORM071074
Első szerző:Mihály Johanna (biológus, vegyész)
Cím:Transient receptor potential vanilloid-3 regulates inflammatory actions of human epidermal keratinocytes / Mihaly J., Vasas N., Angyal A., Payer E., Kistamas K., Nanasi P., Szollosi A., Biro T.
Megjegyzések:Several members of the vanilloid subfamily of transient receptor potential (TRPV) channels are expressed in the skin and play important roles in regulating cutaneous homeostasis. Our aim was to study the expression of TRPV3 ion channel and to investigate its role in normal human epidermal keratinocytes (NHEK).Carvacrol (CRV), a naturally occurring monoterpenoid TRPV3 agonist was administered on NHEKs. Cell viability and proliferation were determined by MTT- and CyQuant-assays, respectively. Gene expression and protein release were assessed by RT-qPCR, Western blot, indirect immuno labeling and ELISA, whereas functional activity of TRPV3 was investigated by whole-cell patch-clamp technique and Fluo-4 AM-based Ca2+-measurement. Selective gene silencing of TRPV3 was performed by RNAi.First, we confirmed that TRPV3 is indeed functionally active on NHEKs. Moreover, its activation by CRV decreased viability and proliferation of keratinocytes in a dose-dependent manner. Importantly, we could also show that expression of certain pro-inflammatory cytokines (interleukin [IL]-1?, IL-6, IL-8 and tumor necrosis factor-?) were upregulated upon CRV treatment in a TRPV3-dependent manner. Furthermore, we also found that silencing of TRPV3 expression was able to efficiently abrogate the aforementioned pro-inflammatory response as well as CRV-induced release of IL-6 and IL-8.Our results strongly argue for that TRPV3 plays a pivotal role in regulating inflammatory actions of epidermal keratinocytes. Antagonizing its activity could therefore be a promising novel therapeutic tool in the management of certain cutaneous inflammatory diseases e.g. in atopic dermatitis.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idézhető absztrakt
pro-inflammatory model systems
Megjelenés:Journal of Investigative Dermatology 135 : Suppl. 2 (2015), p. S44. -
További szerzők:Molnárné Vasas Nikolett (1987-) (élettanász) Angyal Adrienn Páyer Edit (1982-) Kistamás Kornél (1986-) (biológus) Nánási Péter Pál (1956-) (élettanász) Szöllősi Attila Gábor (1982-) (élettanász) Bíró Tamás (1968-) (élettanász) (absztraktok)
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