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001-es BibID:BIBFORM071241
Első szerző:Pataki Emese (molekuláris biológus)
Cím:Interspecific complementation analysis between Schizosaccharomyces pombe and Candida albicans / Emese Pataki, Mátyás Sipiczki, Ida Miklós
ISSN:1217-8950 1588-2640
Megjegyzések:Candida albicans is the most common dimorphic human fungal pathogen. The pathogenesis of itsinfection is poorly understood. A recent study showed that Cas5 played an important role in thepathogenesis of Candida albicans infections and it revealed that Cas5 is required for expression ofcaspofungin-inducible genes, many of which had connection to cell wall integrity. Since defense ofthe host immune system based on the recognition of conserved molecular patterns of the fungal cellwall, deficiencies in the cell wall structure might promote the recognition and elimination ofCandida albicans by host immune cells. Cas5+ encodes a zink-finger transcription factor that ispartially homologous with rsv1+ gene of Schizosaccharomyces group. Based on the sequencesimilarity it is presumable, that rsv1 and Cas5 have the same function. To prove their functionalhomology, we started the interspecific complementation analysis. Namely, we amplified the Cas5+gene by PCR and cloned into S. pombe nmt promoter containing overexpression vector. This DNAconstruction was transformed into the S. pombe rsv1 mutant strain. The characterization and thecaspofungin sensitivity of the transformants are in progress.
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica 62 : Suppl. (2015), p. 197. -
További szerzők:Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
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