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001-es BibID:BIBFORM071244
Első szerző:Pataki Emese (molekuláris biológus)
Cím:rsv1 gene has functional homology in the Schizosaccharomyces species / Emese Pataki, Ida Miklos, Matthias Sipiczki
ISSN:1217-8950 1588-2640
Megjegyzések:Transcriptional regulators are involved in the regulation of gene expression. They are usually boundto the special sites of DNA sequence and hereby allow for a sophisticated response to multipleconditions in the environment. One of the transcriptional regulatory proteins is the rsv1, which ispartly characterised in Sch. pombe. It is a negative regulator of cellular carbohydrate metabolicprocess. It encodes a zinc finger protein, which is required for cell viability in stationary phaseinduced by glucose exhaustion. Cells lacking rsv1+ are viable and the gene is regulated by pka1. [1]The four fission yeast species form an early-branching clade among the Ascomycete. One of them isthe Sch. pombe, which is a well-known model organism. Its relatives, such as Sch. japonicus, Sch.octosporus or Sch. cryophilus can also be attractive models of the genetic studies. We wanted to getinformation about the role of Sch. octosporus rsv1 gene. As there is not rsv1 mutant strain at thismoment in this species, its characterisation is impossible. Therefore, we used the quantification ofsequence similarity and interspecific complementation analysis to prove the function of Sch.octosporus rsv1 gene. The BLASTp analysis suggested, that SOCG_03341 gene must be the rsv1gene. However, sequence-based functional predictions are reliable, not all homologs have the samefunction. Therefore, we cloned the Sch. octosporus rsv1 gene into a Sch. pombe vector, and thisDNA construction was transformed into the Sch. pombe rsv1 mutant strain.The characterisation of the transformants proved the functional homology between the Sch. pombersv1 and Sch. octosporus SOCG_03341 gene.
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica 60 : Suppl. (2013), p. 213. -
További szerzők:Miklós Ida (1962-) (biológus) Sipiczki Mátyás (1948-) (biológus)
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