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001-es BibID:BIBFORM071245
Első szerző:Madar Anett
Cím:Conserved transcriptional regulators in the Schizosaccharomyces group / Anett Madar, Emese Pataki, Ida Miklós, I. J. Holb, M. Sipiczki
Megjegyzések:The Schizosaccharomyces group consists of four species: Sch. pombe, Sch. japonicus, Sch. octosporus, and Sch.cryophilus.Their cells are eukaryotic and haploid, which divide by medial fission. Their genomes have been sequenced(http://www.broadinstitute.org) and the data enable us to compare their genes, proteins and cellular processes. As the fissionyeast cells grow fast, it makes these microorganisms very suitable for breeding. These features give attractive possibilities ofcombining the bioinformatic methods with experimental research. Our research group is interested in studying of celldivision, with special emphasis on cytokinesis and its transcriptional regulation. Therefore, our aim was to gain informationabout the sequence- and functional homology of transcriptional regulators of the cell separation. After bioinformatic analysisof the proteins, we have found two proteins, which showed high degree of homology. Rsv1: Identities=99/167 (59%),Positives=120/167 (71%). Med31: Score = 214.927 (546), Expect=0.0 Identities=98/108 (90%), Positives=104/108 (96%). Inthe med31 protein we have found 37 of 139a.acids, which are constant from yeasts to human. The interspecificcomplementation of these genes has been carried out. Namely, the cloned genes of Sch. octosporus have been transformedinto the mutant cells of Sch. pombe. The investigation of phenotypic features of the transformant cells has revealed functionalhomology between the genes.
Tárgyszavak:Természettudományok Biológiai tudományok előadáskivonat
Megjelenés:A Magyar Mikrobiológiai Társaság 2012. évi Nagygyűlése : Absztraktfüzet. - p. 29.
További szerzők:Pataki Emese (1989-) (molekuláris biológus) Miklós Ida (1962-) (biológus) Holb Imre (1973-) (agrármérnök) Sipiczki Mátyás (1948-) (biológus)
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