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001-es BibID:BIBFORM090656
Első szerző:Baksa Viktória (biomérnök)
Cím:Examination of longitudinal correlation of multi-walled carbon nanotubes (mwcnts), observation of their geno- and cytotoxic effects during corneal regeneration / Baksa Viktória, Kiss Alexandra, Polyák Lenke, Szigeti-Turáni Melinda, Szemán-Nagy Gábor
Megjegyzések:Carbon nanotubes are ultrafine particles in size range of 1-100 nm and organized graphene sheets consisting of one or more layers. Due to their unique structural, physical, and chemical properties, their application in industry is significant. At the same time, they offer promising medical and biological applications, but their safe use in these areas requires reliable toxicological studies. Industrial grade multiwalled carbon nanotubes (MWCNTs) were used in our experiments. Nanoparticles of this type are present in the combustion products of fuels and carbon fiber components used to reinforce some vehicles' plastic elements. Our study aimed to investigate eye injuries caused by car accidents in the presence of carbon nanotubes, using a long-term in vitro regeneration scratch model, where different concentrations (5-100 ?g ml-1), diameter (10-50 nm), and length (0.5 - 30 ?m) MWCNTs were tested for toxic effects. Our research was carried out on human limbal (HuLi) transient amplifying cells. Near-infrared time-lapse video microscopy was used to monitor the effect of nanotubes on the regeneration process. The images thus obtained were quantified by digital image analysis. Examination of chromatin condensation intermediates provides information on genotoxicity. The examination of the chromosome structures is made possible by fluorescence staining using a microscope. Our studies have confirmed that these nanoparticles can enter the wound during exposure to ocular injuries where, depending on the concentration, they can cause poor healing of the cornea. By their physical presence, they can induce cell lawn assembly and partial inhibition of cell proliferation; and extend the time of division compared to control samples. In most cases, MWCNTs prevented the formation of final metaphase chromosomes during nuclear condensation. The prolonged regeneration and slower, scarred healing of the injured cell lawn and the disruption of the chromatin condensation process may be further enhanced by the aggregation phenomenon of carbon nanotube length. In our experience, the length of carbon nanotubes is proportional to the size of their micro-aggregates. However, increased aggregate sizes (300-9000 ?m2) showed less genotoxic and cytotoxic effects than their shorter variants, where aggregates fell in the range of 250-350 ?m2.
Tárgyszavak:Természettudományok Biológiai tudományok előadáskivonat
Megjelenés:4th National Conference of Young Biotechnologists "FIBOK 2020" online conference : abstract book / Ed. by Tünde Pusztahelyi, Levente Czeglédi, Éva Domokos-Szabolcsy, Tamás Emri. - p. 57.
További szerzők:Kiss Alexandra (1994-) (gyógyszer-biotechnológus) Polyák Lenke (1998-) (Biológus) Turáni Melinda (1985-) (biológus) Szemán-Nagy Gábor (1975-) (biológia tanár-molekuláris biológus)
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat
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