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001-es BibID:	BIBFORM030251
035-os BibID:	(Scopus)17544396717 (WoS)000182446700007
Első szerző:	Bányász Tamás (élettanász)
Cím:	Endocardial versus epicardial differences in L-type calcium current in canine ventricular myocytes studied by action potential voltage clamp / Tamás Bányász, László Fülöp, János Magyar, Norbert Szentandrássy, András Varró, Péter P. Nánási
Dátum:	2003
ISSN:	0008-6363
Megjegyzések:	Objectives: The aim of the present study was to assess and compare the dynamics of L-type Ca2+ current (I-Ca.L) during physiologic action potential (AP) in canine ventricular cardiomyocytes of epicardial (EPI) and endocardial (ENDO) origin. Methods: I-Ca.L was recorded on cells derived from the two regions of the heart using both AP voltage clamp and conventional whole cell voltage clamp techniques. Results: AP voltage clamp experiments revealed that the decay of I-Ca.L is monotonic during endocardial AP, whereas the current is double-peaked (displaying a second rise) during epicardial AP. The amplitude of the first peak was significantly greater in ENDO (-4.6+/-0.8 pA/pF) than in EPI cells (-2.8+/-0.3 pA/pF). Application of epicardial APs as command pulses to endocardial cells yielded double-peaked I-Ca.L profiles, and increased the net charge entry carried by I-Ca.L during the AP from 0.187+/-0.059 to 0.262+/-0.056 pC/pF (n=5, P<0.05). No differences were observed in current densities and inactivation kinetics of I-Ca.L between EPI and ENDO cells when studied under conventional voltage clamp conditions. Nisoldipine shortened action potentials and eliminated the dome of the epicardial AP. Conclusion: I-Ca.L was shown to partially inactivate before and deactivate during phase-1 repolarization and reopening of these channels is responsible for the formation of the dome in canine EPI cells. The transmural differences in the profile of I-Ca.L could be well explained with differences in AP configuration. (C) 2003 European Society of Cardiology. Published by Elsevier Science B.V. All rights reserved.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban egyetemen (Magyarországon) készült közlemény
Megjelenés:	Cardiovascular Research. - 58 : 1 (2003), p. 66-75. -
További szerzők:	Fülöp László (1976-) (kardiológus) Magyar János (1961-) (élettanász) Szentandrássy Norbert (1976-) (élettanász) Varró András (1954-) (farmakológus, klinikai farmakológus) Nánási Péter Pál (1956-) (élettanász)
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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