CCL

Összesen 2 találat.
#/oldal:
Részletezés:
Rendezés:

1.

001-es BibID:BIBFORM049047
Első szerző:Szalóki Melinda (vegyész)
Cím:Binding of leachable components of polymethyl methacrylate (PMMA) and peptide on modified SPR chip / M. Szalóki, G. Vitályos, J. Hársfalvi, Cs. Hegedűs
Dátum:2013
ISSN:1757-8981 1757-899X
Megjegyzések:Abstract. Many types of polymers are often used in dentistry, which may cause allergic reaction, mainly methyl methacrylate allergy due to the leachable, degradable components of polymerized dental products. The aim of this study was to investigate the interaction between the leachable components of PMMA and peptides by Fourier-transform Surface Plasmon Resonance (FT SPR). In our previous work binding of oligopeptides (Ph.D.-7 and Ph.D.-12 Peptide Library Kit) was investigated to PMMA surface by phage display technique. It was found that oligopeptides bounded specifically to PMMA surface. The most common amino acids were leucine and proline inside the amino acids sequences of DNA of phages. The binding of haptens, as formaldehyde and methacrylic acid, to frequent amino acids was to investigate on the modified gold SPR chip. Self assembled monolayer (SAM) modified the surface of gold chip and ensured the specific binding between the haptens and amino acids. It was found that amino acids bounded to modified SPR gold and the haptens bounded to amino acids by creating multilayer on the chip surface. By the application of phage display and SPR modern bioanalytical methods the interaction between allergens and peptides can be investigated.
Tárgyszavak:Orvostudományok Klinikai orvostudományok konferenciacikk
folyóiratcikk
phage display
FT-SPR
PMMA
dental allergens
Doktori iskola
Megjelenés:IOP Conference Series: Materials Science and Engineering. - 47 (2013), p. 1-6. -
További szerzők:Vitályos Géza (1970-) (egyetemi tanársegéd, fogszakorvos) Hársfalvi Jolán (1949-) (klinikai biokémikus) Hegedűs Csaba (1953-) (fogszakorvos)
Pályázati támogatás:TÁMOP-4.2.2/B-10/1-2010-0024
TÁMOP
Fogorvostudományi Doktori Iskola
Internet cím:Szerző által megadott URL
DOI
Intézményi repozitóriumban (DEA) tárolt változat
Borító:

2.

001-es BibID:BIBFORM044376
Első szerző:Vitályos Géza (egyetemi tanársegéd, fogszakorvos)
Cím:Binding of oligopeptide to polymethyl methacrylate surface / G. Vitalyos, M. Szaloki, J. Harsfalvi, C. Hegedus
Dátum:2012
ISSN:0022-0345
Megjegyzések:Objective: Polymers (Polymethyl methacrylate; PMMA) are one the most frequently used material in dentistry, which is often causingallergic reactions. The aim of our study was to investigate peptide structures bound to PMMA in order to deduct molecules may bind toPMMA and responsible for allergic reactions.Method: The investigated heat cured polymethyl methacrylate was Orthocryl (Dentaurum, Germany). We used phage display technologyto select peptides on PMMA surface. The phage work was carried out by linear peptide library. Ph.D.-7T M and Ph.D.-12T M Peptide LibraryKits (Biolabs, England) were based on a combinatorial library of random peptide 7-mers and random peptide 12-mers fused to a minorcoat protein of M13 Phage respectively. The phage selection was carried out five times on the PMMA surface. C olonies of the third roundsof each phage selection were used to isolate DNS for amino acid sequence determination. The binding of the selected peptides wasquantitated by ELISA and by calculation of binding constants.Result: Efficacy of the phage selections resulted in 2.33x107 phages/ml on the titer plate. At the estimation of binding affinities of theselected phages it was found that the phages bound to the surface with saturation kinetic. The amino acid sequence of the peptide insertsof selected phages showed that mostly apolar, non-aromatic amino acid occurred among them the proline is the most frequent. TheNVQTRQPEKPVL sequence gave a relative low E-value at the comparison of protein data bank.C onclusion: Oligopeptides bound to the PMMA surface and among PMMA specific phages the proline and leucine is the most oftenoccurred amino acid. The NVQTRQPEKPVL sequence may occur in that haptens or immunmediators which can be responsible for theallergic reaction.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idézhető absztrakt
dental allergy
Megjelenés:Journal of Dental Research. - 91 : B (2012), p. 3210. -
További szerzők:Szalóki Melinda (1981-) (vegyész) Hársfalvi Jolán (1949-) (klinikai biokémikus) Hegedűs Csaba (1953-) (fogszakorvos)
Pályázati támogatás:TÁMOP-4.2.1/B-09/1/KONV-2010-0007
TÁMOP
Internet cím:Szerző által megadott URL
Intézményi repozitóriumban (DEA) tárolt változat
Borító:
Rekordok letöltése1