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001-es BibID:BIBFORM031960
Első szerző:Fehér Enikő (molekuláris biológus, mikrobiológus)
Cím:Comparison of diversity of torque teno virus 1 in different mucosal tissues and disorders / Fehér Enikő, Kardos Gábor, Gáll Tamás, Kis Andrea, Gergely Lajos, Szarka Krisztina
Megjegyzések:Diversity of TTV1 was assessed in the head and neck region in patients with potentially malignant (oral lichen planus, oral leukoplakia) and malignant lesions (oral and laryngeal squamous cell cancers) and was compared to that found in the uterine cervix (cervical atypia and cervical cancer) by directly sequencing the NG061-063 segment of ORF1. These sequences were classified by the formerly used genogroupgenotype system as well as by the newly accepted species classification by aligning with the corresponding region of the type sequences of the 29 TTV species. All sequences obtained during the study clustered together with the TTV1 type sequence; to express diversity within TTV1, genotypes and subtypes of the former classification were used.The commonest subtypes were 2c followed by 2b, 1a and 1b. Subtypes 2b and 2c were evenly distributed among cervical samples; subtype 1a was more frequent in patients with cervical atypia or cancer. Subtypes 2c was more frequent than 2b in head and neck lesions. In conclusion, genotype and even subtype distribution may be important in association with diseases, therefore using this classification for characterization of intraspecies diversity of TTV1 is proposed.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény hazai lapban
Megjelenés:Acta Microbiologica et Immunologica Hungarica 58 : 4 (2011), p. 319-337. -
További szerzők:Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Gáll Tamás (1982-) (molekuláris biológus, mikrobiológus) Kis Andrea (1981-) (molekuláris biológus, mikrobiológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
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001-es BibID:BIBFORM047703
035-os BibID:PMID:23649705
Első szerző:Gáll Tamás (molekuláris biológus, mikrobiológus)
Cím:Genomic differences in the background of different severity in juvenile-onset respiratory papillomatoses associated with human papillomavirus type 11 / Tamás Gáll, Andrea Kis, Tímea Zsófia Tatár, Gábor Kardos, Lajos Gergely, Krisztina Szarka
Megjegyzések:This study aimed to compare complete genome sequences of human papillomavirus (HPV) type 11 from two solitary papillomas (considered minimally aggressive), two moderately (six and nine episodes) and two highly aggressive (30 and 33 episodes) juvenile-onset respiratory papillomatoses. Genomic regions were sequenced using the Sanger method; sequences were compared to available GenBank genomes. Activity of the long control region (LCR) was assessed in HEp-2 cell line using luciferase assays and compared to that of the reference (GenBank Accession Number M14119). Site-directed mutagenesis was performed to confirm the association of polymorphisms with differences in LCR activity. Eleven alterations resulted in amino acid changes in different open reading frames. A72E in E1 and Q86K in E2 proteins were exclusively present in a moderately aggressive disease, L1 alterations A476V and S486F were unique to a severe papillomatosis. HPV11s in both solitary papillomas had identical LCRs containing a T7546C polymorphism, which strongly attenuated LCR activity, as confirmed by site-directed mutagenesis. This strong attenuator polymorphism was also present in the other four genomes showing significantly higher activities, but in these other alterations with demonstrable but statistically not significant attenuating (A7413C, 7509 T deletion) or enhancing (C7479T, T7904A) effect on transactivating potential (as demonstrated by site-directed mutagenesis) were also detected. LCR activities corresponded well to severity, excepting the highly aggressive papillomatosis with the L1 alterations. Presence of intratypic variants cannot explain differences in severity of respiratory papillomatoses associated with HPV11; virulence seems to be determined by the interaction of multiple genetic differences.
Tárgyszavak:Orvostudományok Klinikai orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Medical Microbiology and Immunology. - 202 : 5 (2013), p. 353-363. -
További szerzők:Kis Andrea (1981-) (molekuláris biológus, mikrobiológus) Tatár Tímea Zsófia Kardos Gábor (1974-) (szakorvos, klinikai mikrobiológus) Gergely Lajos (1940-) (szakorvos, klinikai mikrobiológus) Szarka Krisztina (1971-) (molekuláris biológus, mikrobiológus)
Pályázati támogatás:OTKA 73145
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