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001-es BibID:	BIBFORM016491
Első szerző:	Vig Attila
Cím:	Development and characterization of a FIA system for selective assay of l-ascorbic acid in food samples / Attila Vig, Attila Igloi, Nora Adanyi, Gyongyi Gyemant, Csaba Csutoras and Attila Kiss
Dátum:	2010
ISSN:	1615-7591
Megjegyzések:	An amperometric detector and an enzymatic reaction were combined for the measurement of l-ascorbic acid. The enzyme cell (containing immobilized ascorbate oxidase) was connected to a flow injection analyzer (FIA) system with a glassy carbon electrode as an amperometric detector. During optimization and measurements two sample injectors were used, one before and one after the enzyme cell, thus eliminating the background interferences. Subtraction of the signal area given in the presence of enzyme from the one given in the absence of enzyme was applied for measuring analyte concentrations and calibration at 400 mV. Analysis capacity of system is 25 samples/hour. The relative standard deviation (RSD) was below 5% (5 times repeated, 400 mu mol/L conc.), linearity up to 400 mu mol/L, limit of detection (LOD) 5 mu mol/L, fitting of calibration curve in 25-400 mu mol/L range was R (2) = 0.99.
Tárgyszavak:	Természettudományok Kémiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Biosensor Ascorbic acid Amperometry Glassy carbon Flow injection analysis biosensor oxidase
Megjelenés:	Bioprocess and Biosystems Engineering. - 33 : 8 (2010), p. 947-952. -
További szerzők:	Iglói Attila (1971-) (biológus-mikrobiológus) Adanyi Nóra Gyémánt Gyöngyi (1960-) (vegyész) Csutorás Csaba Kiss Attila
Internet cím:	DOI Intézményi repozitóriumban (DEA) tárolt változat
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