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001-es BibID:	BIBFORM020261
Első szerző:	Kaszanyitzky J. Éva
Cím:	Staphylococci isolated from animals and food with phenotypically reduced susceptibility to beta-lactamase-resistant beta-lactam antibiotics / Kaszanyitzky É. J., Egyed Zs., Jánosi Sz., Keserű J., Gál Zs., Szabó I., Veres Z., Somogyi P.
Dátum:	2004
ISSN:	0236-6290
Megjegyzések:	The antibiotic resistance pattern of 1921 Staphylococcus strains isolatedfrom animals and food within the last two years were examined using diffusiontests. Among them there were only 35 strains of S. aureus having an inhibitionzone diameter of 15 mm or less, and 4 strains of coagulase-negative staphylococci(CNS) having a zone diameter of 18 mm or less to 1-?g oxacillin disk. These 39strains were examined also by E-test to oxacillin and for the detection of the mecAgene by PCR in order to determine whether they might be real methicillinresistantstaphylococci. Among the 39 strains there were only two that were susceptibleto penicillin by disk diffusion method; however, further examination bythe penicillinase test showed that they produced beta-lactamase. While 19 (15 S.aureus, 4 CNS) strains were resistant and 7 strains were intermediate to oxacillinin disk diffusion test, the E-test gave 8 resistant and 5 intermediate results. Six outof the 8 oxacillin-resistant strains examined by disk diffusion and E-test harbouredthe mecA gene. Thus only 6 out of the examined 1921 strains proved to bemecA positive. These methicillin-resistant, mecA-positive strains (5 of the S.aureus strains and 1 of the S. epidermidis) originated from two dairy herds. Theresults prove that methicillin-resistant S. aureus (MRSA) strains in animals arereally rare in Hungary. Eighteen strains were chosen and screened for minimal inhibitoryconcentration (MIC) of oxacillin with or without clavulanic acid or sulbactam,and three of them produced methicillinase enzyme.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény hazai lapban methicillin-resistant Staphylococcus aureus (MRSA) methicillin- resistant coagulase negative staphylococci (CNS) borderline resistance
Megjelenés:	Acta Veterinaria Hungarica. - 52 : 1 (2004), p. 7-17. -
További szerzők:	Egyed Zsuzsanna Jánosi Szilárd Keserű Judit (1976-) (molekuláris genetikus) Gál Zsuzsanna (1959-) (vegyész) Szabó István (1950-) (sejtbiológus, molekuláris genetikus) Veres Zoltán Somogyi Péter
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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001-es BibID:	BIBFORM028739
Első szerző:	Keserű Judit (molekuláris genetikus)
Cím:	Identification of beta-lactamases in human and bovine isolates of Staphylococcus aureus strains having borderline resistance to penicillinase-resistant penicillins (PRPs) with proteomic methods / Keserű Judit Szilvia, Szabó István, Gál Zsuzsanna, Orietta Massidda, Marina Mingoia, Kaszanyitzky Éva, Jánosi Szilárd, Hulvely Julianna, Csorba Attila, Buzás Krisztina, Hunyadi-Gulyás Éva, Medzihradszky F. Katalin, Biró Sándor
Dátum:	2011
ISSN:	0378-1135
Megjegyzések:	Methicillin and oxacillin-hydrolyzing enzymes of 6 borderline methicillin-resistant and 1 methicillin-resistant Staphylococcus aureus strains isolated from human clinical samples and 4 borderline methicillin-resistant S. aureus strains isolated from bovine mastitis were investigated. As previous studies suggested the involvement of an additional enzyme besides the penicillinase BlaZ in the determination of borderline resistance, we analyzed the expressed extracellular and membrane-bound beta-lactamases with 2-D gel electrophoresisand mass spectrometry. Our analysis showed that the penicillin-hydrolyzing BlaZ alone was responsible for the hydrolysis of both methicillin and oxacillin. All supernatant and membrane fractions contained the same enzyme with slight sequence variations. The size and pI of the proteins were also variable, probably due to spontaneous hydrolysis and/or posttranslational modifications. Interestingly, we found also cytotoxins and other virulence factors in some nitrocefin-hydrolyzing dots, suggesting that those proteins might have a role in the reduction of local antibiotic concentration.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Staphylococcus aureus Borderline methicillin-resistance Membrane-bound beta-lactamase Penicillinase PRPs-hydrolyzing enzyme Proteomics
Megjelenés:	Veterinary Microbiology. - 147 : 1-2 (2011), p. 96-102. -
További szerzők:	Szabó István (1950-) (sejtbiológus, molekuláris genetikus) Gál Zsuzsanna (1959-) (vegyész) Massidda, Orietta Mingoia, Marina Kaszanyitzky J. Éva Jánosi Szilárd Hulvely Julianna Csorba Attila Buzás Krisztina Hunyadi-Gulyás Éva Medzihradszky-Fölkl Katalin Biró Sándor (1949-) (molekuláris genetikus)
Pályázati támogatás:	ETT 395/KO/03 Egyéb
Internet cím:	Szerző által megadott URL DOI Intézményi repozitóriumban (DEA) tárolt változat
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