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001-es BibID:BIBFORM099535
Első szerző:Bartáné Tóth Beáta (molekuláris biológus)
Cím:Regulatory modules of human thermogenic adipocytes : functional genomics of large cohort and Meta-analysis derived marker-genes / B. Tóth Beáta, Barta Zoltán, Barta Ákos Barnabás, Fésüs László
Megjegyzések:Background Recently, ProFAT and BATLAS studies identified brown and white adipocytes marker genes based on analysis of large databases. They offered scores to determine the thermogenic status of adipocytes using the gene-expression data of these markers. In this work, we investigated the functional context of these genes. Results Gene Set Enrichment Analyses (KEGG, Reactome) of the BATLAS and ProFAT marker-genes identified pathways deterministic in the formation of brown and white adipocytes. The collection of the annotated proteins of the defined pathways resulted in expanded white and brown characteristic protein-sets, which theoretically contain all functional proteins that could be involved in the formation of adipocytes. Based on our previously obtained RNA-seq data, we visualized the expression profile of these proteins coding genes and found patterns consistent with the two adipocyte phenotypes. The trajectory of the regulatory processes could be outlined by the transcriptional profile of progenitor and differentiated adipocytes, highlighting the importance of suppression processes in browning. Protein interaction network-based functional genomics by STRING, Cytoscape and R-Igraph platforms revealed that different biological processes shape the brown and white adipocytes and highlighted key regulatory elements and modules including GAPDH-CS, DECR1, SOD2, IL6, HRAS, MTOR, INS-AKT, ERBB2 and 4-NFKB, and SLIT-ROBO-MAPK. To assess the potential role of a particular protein in shaping adipocytes, we assigned interaction network location-based scores (betweenness centrality, number of bridges) to them and created a freely accessible platform, the AdipoNET (https//, to conveniently use these data. The Eukaryote Promoter Database predicted the response elements in the UCP1 promoter for the identified, potentially important transcription factors (HIF1A, MYC, REL, PPARG, TP53, AR, RUNX, and FoxO1). Conclusion Our integrative approach-based results allowed us to investigate potential regulatory elements of thermogenesis in adipose tissue. The analyses revealed that some unique biological processes form the brown and white adipocyte phenotypes, which presumes the existence of the transitional states. The data also suggests that the two phenotypes are not mutually exclusive, and differentiation of thermogenic adipocyte requires induction of browning as well as repressions of whitening. The recognition of these simultaneous actions and the identified regulatory modules can open new direction in obesity research.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:BMC Genomics. - 22 : 1 (2021), p. 1-21. -
További szerzők:Barta Zoltán (1967-) (biológus, zoológus) Barta Ákos Barnabás Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:GINOP-2.3.2-15-2016-00006
Internet cím:Szerző által megadott URL
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001-es BibID:BIBFORM013410
Első szerző:Bartáné Tóth Beáta (molekuláris biológus)
Cím:Ecological studies of ectomycorrhizal fungi : an analysis of survey methods / Beáta B. Tóth, Zoltan Barta
Megjegyzések:In this paper, by reviewing ecological studies ofectomycorrhizal fungi where both fruiting bodies andmycorrhizal root tips were simultaneously surveyed, weinvestigate whether the diversity data obtained by the twomethods leads to similar conclusions about the underlyingecological processes of interest. Despite discrepancies inidentifying species, we found that both survey methodsidentified similar responses by ectomycorrhizal fungalcommunities to experimental manipulations, successionalchanges and environmental disturbances (exceptions areshort-term or low-level disturbances). By analysing theresults of the reviewed studies, we found a positiverelationship to exist between fungal species richness and(i) the host plant age and (ii) the number of putative hostplant species, independently of the applied survey method.Of the methodological variables, only the number of soilsamples (for the below-ground approach) and the durationof the study (for the above-ground approach) have asignificant effect on the EMF species richness, with speciesrichness increasing with both. Our investigation also showsthat in 73% of the reviewed studies (27 out of 37) a greaterspecies richness was found by fruiting body surveys thanby methods based on sampling of the root tips. Based onthese findings, we argue for the continuation of fruitingbody surveys in order to gain rapid and still valuableinformation on ecosystems over a wide spatial and temporalrange and strongly recommend their use in long-termecosystem monitoring projects.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Fungal diversity
Ecosystem processes
Megjelenés:Fungal Diversity. - 45 : 1 (2010), p. 3-19. -
További szerzők:Barta Zoltán (1967-) (biológus, zoológus)
Internet cím:DOI
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