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001-es BibID:BIBFORM064290
Első szerző:Lopandic, Ksenija
Cím:Genotypic and phenotypic evolution of yeast interspecies hybrids during high-sugar fermentation / Ksenija Lopandic, Walter P. Pfliegler, Wolfgang Tiefenbrunner, Helmut Gangl, Matthias Sipiczki, Katja Sterflinger
Dátum:2016
ISSN:0175-7598
Megjegyzések:The yeasts of the Saccharomyces genus exhibit a low pre-zygotic barrier and readily form interspecies hybrids. Following the hybridization event, the parental genomes undergo gross chromosomal rearrangements and genome modifications that may markedly influence the metabolic activity of descendants. In the present study, two artificially constructed hybrid yeasts (Saccharomyces cerevisiae x Saccharomyces uvarum and S. cerevisiae x Saccharomyces kudriavzevii) were used in order to evaluate the influence of high-sugar wine fermentation on the evolution of their genotypic and phenotypic properties. It was demonstrated that the extent of genomic modifications differs among the hybrids and their progeny, but that stress should not always be a generator of large genomic disturbances. The major genome changes were observed after meiosis in the F1 segregants in the form of the loss of different non-S. cerevisiae chromosomes. Under fermentation condition, each spore clone from a tetrad developed a mixed population characterized by different genotypic and phenotypic properties. The S. cerevisiae x S. uvarum spore clones revealed large modifications at the sequence level of the S. cerevisiae sub-genome, and some of the clones lost a few additional S. cerevisiae and S. uvarum chromosomes. The S. cerevisiae x S. kudriavzevii segregants were subjected to consecutive loss of the S. kudriavzevii markers and chromosomes. Both the hybrid types showed increased ethanol and glycerol production as well as better sugar consumption than their parental strains. The hybrid segregants responded differently to stress and a correlation was found between the observed genotypes and fermentation performances.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Applied Microbiology And Biotechnology. - 100 : 14 (2016), p. 6331-6343. -
További szerzők:Pfliegler Valter Péter (1986-) (molekuláris biológus) Tiefenbrunner, Wolfgang Gangl, Helmut Sipiczki Mátyás (1948-) (biológus) Sterflinger, Katja
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001-es BibID:BIBFORM055415
Első szerző:Pfliegler Valter Péter (molekuláris biológus)
Cím:Generation of new genotypic and phenotypic features in artificial and natural yeast hybrids / Walter P. Pfliegler, Lea Atanasova, Edina Karanyicz, Matthias Sipiczki, Ursula Bond, Irina S. Druzhinina, Katja Sterflinger, Ksenija Lopandic
Dátum:2014
ISSN:1330-9862 1334-2606
Megjegyzések:Evolution and genome stabilization have mostly been studied on the Saccharomyceshybrids isolated from natural and alcoholic fermentation environments. Genetic and phenotypicproperties have usually been compared to the laboratory and reference strains, as thetrue ancestors of the natural hybrid yeasts are unknown. In this way the exact impact ofdifferent parental fractions on the genome organization or metabolic activity of the hybridyeasts is difficult to resolve completely. In the present work the evolution of geno- andphenotypic properties is studied in the interspecies hybrids created by the cross-breedingof S. cerevisiae with S. uvarum or S. kudriavzevii auxotrophic mutants. We hypothesized thatthe extent of genomic alterations in S. cerevisiae ? S. uvarum and S. cerevisiae ? S. kudriavzeviishould affect the physiology of their F1 offspring in different ways. Our results, obtainedby amplified fragment length polymorphism (AFLP) genotyping and karyotypinganalyses, showed that both subgenomes of the S. cerevisiae x S. uvarum and of S. cerevisiae ?S. kudriavzevii hybrids experienced various modifications. However, the S. cerevisiae ? S.kudriavzevii F1 hybrids underwent more severe genomic alterations than the S. cerevisiae ?S. uvarum ones. Generation of the new genotypes also influenced the physiological performancesof the hybrids and the occurrence of novel phenotypes. Significant differencesin carbohydrate utilization and distinct growth dynamics at increasing concentrations ofsodium chloride, urea and miconazole were observed within and between the S. cerevisiae? S. uvarum and S. cerevisiae ? S. kudriavzevii hybrids. Parental strains also demonstrateddifferent contributions to the final metabolic outcomes of the hybrid yeasts. A comparisonof the genotypic properties of the artificial hybrids with several hybrid isolates from thewine-related environments and wastewater demonstrated a greater genetic variability ofthe S. cerevisiae ? S. kudriavzevii hybrids. Saccharomyces cerevisiae ? S. uvarum artificial andnatural hybrids showed considerable differences in osmolyte tolerance and sensitivity tomiconazole, whereas the S. cerevisiae ? S. kudriavzevii hybrids exhibited differences also inmaltotriose utilization. The results of this study suggest that chromosomal rearrangementsand genomic reorganizations as post-hybridization processes may affect the phenotypicproperties of the hybrid progeny substantially. Relative to their ancestors, the F1 segregants may generate different phenotypes, indicating novel routes of evolution in responseto environmental growth conditions.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Megjelenés:Food Technology and Biotechnology. - 52 : 1 (2014), p. 46-57. -
További szerzők:Atanasova, Lea Karanyicz Edina Sipiczki Mátyás (1948-) (biológus) Bond, Ursula Druzhinina, Irina S. Sterflinger, Katja Lopandic, Ksenija
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3.

001-es BibID:BIBFORM090571
035-os BibID:(cikkazonosító)362
Első szerző:Selbmann, Laura
Cím:Shed Light in the DaRk LineagES of the Fungal Tree of Life-STRES / Laura Selbmann, Zsigmond Benkő, Claudia Coleine, Sybren de Hoog, Claudio Donati, Irina Druzhinina, Tamás Emri, Cassie L. Ettinger, Amy S. Gladfelter, Anna A. Gorbushina, Igor V. Grigoriev, Martin Grube, Nina Gunde-Cimerman, Zsolt Ákos Karányi, Beatrix Kocsis, Tania Kubressoian, Ida Miklós, Márton Miskei, Lucia Muggia, Trent Northen, Monika Novak-Babič, Christa Pennacchio, Walter P. Pfliegler, Istvàn Pòcsi, Valeria Prigione, Meritxell Riquelme, Nicola Segata Julia Schumacher, Ekaterina Shelest, Katja Sterflinger, Donatella Tesei, Jana M. U'Ren, Giovanna C. Varese, Xabier Vázquez-Campos, Vania A. Vicente, Emanuel M. Souza, Polona Zalar, Allison K. Walker, Jason E. Stajich
Dátum:2020
ISSN:2075-1729
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
folyóiratcikk
Megjelenés:Life. - 10 : 12 (2020), p. 1-13. -
További szerzők:Benkő Zsigmond (1961-) (molekuláris biológus, genetikus) Coleine, Claudia de Hoog, Sybren Donati, Claudio Druzhinina, Irina S. Emri Tamás (1969-) (biológus) Ettinger, Cassie L. Gladfelter, Amy S. Gorbushina, Anna Grigoriev, Igor V. Grube, Martin Gunde-Cimerman, Nina Karányi Zsolt (1961-) (biostatisztikus, bioinformatikus) Kocsis Beatrix (1988-) (molekuláris biológus) Kubressoian, Tania Miklós Ida (1962-) (biológus) Miskei Márton (1978-) (molekuláris biológus, genetikus) Muggia, Lucia Northen, Trent Novak-Babič, Monika Pennacchio, Christa Pfliegler Valter Péter (1986-) (molekuláris biológus) Pócsi István (1961-) (vegyész) Prigione, Valeria Riquelme, Meritxell Segata, Nicola Schumacher, Julia Shelest, Ekaterina Sterflinger, Katja Tesei, Donatella U'Ren, Jana M. Varese, Giovanna C. Vázquez-Campos, Xabier Vicente, Vania A. Souza, Emanuel M. Zalar, Polona Walker, Allison K. Stajich, Jason E.
Pályázati támogatás:DE-AC02-05CH11231
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