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001-es BibID:	BIBFORM063055
Első szerző:	Gonda Sándor (gyógyszerész)
Cím:	Effects of N source concentration and NH4+/NO3- ratio on phenylethanoid glycoside pattern in tissue cultures of Plantago lanceolata L.: A metabolomics driven full-factorial experiment with LC-ESI-MS3 / Sándor Gonda, Attila Kiss-Szikszai, Zsolt Szűcs, Csaba Máthé, Gábor Vasas
Dátum:	2014
ISSN:	0031-9422
Megjegyzések:	Tissue cultures of a medicinal plant, Plantago lanceolata L. were screened for phenylethanoid glycosides (PGs) and other natural products (NPs) with LC?ESI?MS 3 . The effects of N source concentration and NH + 4 /NO 3 ratio were evaluated in a full-factorial (FF) experiment. N concentrations of 10, 20, 40 and 60 mM, and NH + 4 /NO 3 ratios of 0, 0.11, 0.20 and 0.33 (ratio of NH + 4 in total N source) were tested. Several peaks could be identified as PGs, of which, 16 could be putatively identified from the MS/MS/MS spectra. N source concentration and NH4+ /NO3- ratio had significant effects on the metabolome, their effects on individual PGs were different despite these metabolites were of the same biosynthethic class. Chief PGs were plantamajoside and acteoside (verbascoside), their highest concentrations were 3.54 ? 0.83% and 1.30 ? 0.40% of dry weight, on media 10(0.33) and 40(0.33), respectively. NH4+ /NO3- ratio and N source concentration effects were examined on a set of 89 NPs. For most NPs, high increases in abundance were observed compared to Murashige?Skoog medium. Abundances of 42 and 10 NPs were significantly influenced by the N source concentration and the NH + 4 /NO 3 ratio, respectively. Optimal media for production of different NP clusters were 10(0), 10(0.11) and 40(0.33). Interaction was observed between NH + 4 /NO 3 ratio and N source concentration for many NPs. It was shown in simulated experiments, that one-factor at a time (OFAT) experimental designs lead to sub-optimal media compositions for production of many NPs, and alternative experimental designs (e.g. FF) should be preferred when optimizing medium N source for optimal yield of NPs. If using OFAT, the N source concentration is to be optimized first, followed by NH4+/NO3- ratio, as this reduces the likeliness of suboptimal yield results.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban Phenylpropanoid glycosides Polyphenolic substances Phenoloid secondary metabolites Data-mining Design of experiments Medicinal plant tissue culture Omics Ammonium Nitrate
Megjelenés:	Phytochemistry. - 106 (2014), p. 44-54. -
További szerzők:	Kiss-Szikszai Attila (1975-) (vegyész) Szűcs Zsolt (1990-) (gyógyszerész) Máthé Csaba (1966-) (biológus) Vasas Gábor (1975-) (biológus-vegyész)
Pályázati támogatás:	PD 112374 OTKA K 81370 OTKA 4.2.2.A-11/1/KONV-2012-0043 TÁMOP 4.2.1/B-09/1/KONV-2010-0007 TÁMOP 4.2.4. A/2-11-1-2012-0001 TÁMOP
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001-es BibID:	BIBFORM120976
Első szerző:	Szűcs Zsolt (gyógyszerész)
Cím:	Production of Polyphenolic Natural Products by Bract-Derived Tissue Cultures of Three Medicinal Tilia spp.: A Comparative Untargeted Metabolomics Study / Zsolt Szűcs, Zoltán Cziáky, László Volánszki, Csaba Máthé, Gábor Vasas, Sándor Gonda
Dátum:	2024
ISSN:	2223-7747
Megjegyzések:	Medicinal plant tissue cultures are potential sources of bioactive compounds. In this study, we report the chemical characterization of the callus cultures of three medicinal Tilia spp. (Tilia cordata, Tilia vulgaris and Tilia tomentosa), along with the comparison to bracts and flowers of the same species. Our aim was to show that calli of Tilia spp. are good alternatives to the calli of T. americana for the production of polyphenols and are better sources of a subset of polyphenolic metabolites, compared to the original organs. Calli were initiated from young bracts and grown on woody plant medium containing 1 mg L?1 2,4-D and 0.1 mg L?1 BAP. For chemical characterization, a quality-controlled untargeted metabolomics approach and the quantification of several bioactive compounds was performed with the use of LC-ESI-MS/MS. While bracts and flowers contained flavonoid glycosides (astragalin, isoquercitrin) as major polyphenols, calli of all species contained catechins, coumarins (fraxin, esculin and scopoletin) and flavane aglyca. T. tomentosa calli contained 5397 ?g g DW?1 catechin, 201 ?g g DW?1 esculin, 218 ?g g DW?1 taxifolin and 273 ?g g DW?1 eriodictyol, while calli from other species contained lower amounts. T. cordata and T. tomentosa flowers were rich in isoquercitrin, containing 8134 and 6385 ?g g DW?1, respectively. The currently tested species contained many of the bioactive metabolites described from T. americana. The production of catechin was shown to be comparable to the most efficient tissue cultures reported. Flowers and bracts contained flavonoid glycosides, including tiliroside, resembling bioactive fractions of T. americana. In addition, untargeted metabolomics has shown fingerprint-like differences among species, highlighting possible chemotaxonomic and quality control applications, especially for bracts.
Tárgyszavak:	Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk linden lime tree plant tissue culture natural products in vitro culture
Megjelenés:	Plants-Basel. - 13 : 10 (2024), p. 1-19. -
További szerzők:	Cziáky Zoltán Volánszki László (1994-) (Gyógyszerész) Máthé Csaba (1966-) (biológus) Vasas Gábor (1975-) (biológus-vegyész) Gonda Sándor (1984-) (gyógyszerész)
Pályázati támogatás:	142146 OTKA 146030 OTKA Bolyai János Ösztöndíj Egyéb ÚNKP-23-5 Egyéb
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