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001-es BibID:BIBFORM104320
Első szerző:Gulyás Andrea
Cím:Epigenome of Malus domestica / Andrea Gulyás, Judit Dobránszki, Erzsébet Kiss, Katalin Posta, Norbert Hidvégi
Megjegyzések:The aim of this study was to examine the epigenomes of two diploid apple scion cultivars from three distinct environments: 1) in vitro shoots maintained for 16 years in tissue culture; 2) in vivo mother trees (20 years old); 3) acclimatized in vitro plants (one year after acclimatization). Using whole-genome bisulfite sequencing (WGBS), the level of DNA methylation was measured in these three biological samples to determine whether an epigenetic footprint was left within the epigenome of apple due to different environments (in vivo mother tree vs. in vitro) or a change in the environment (in vitro culture to acclimatized stage). The study further assessed if the DNA methylation pattern of acclimatized plants mirrored that of the original parental material (i.e., in vivo mother plant vs. one year after acclimatization).
Tárgyszavak:Agrártudományok Növénytermesztési és kertészeti tudományok előadáskivonat
DNA methylation
gene expression
Megjelenés:18th Alps-Adria Scientific Workshop : Alimentation and Agri-environment : Abstract book / (ed.) Kende Zoltán. - p. 60-61. -
További szerzők:Dobránszki Judit (1967-) (biológus) Kiss Erzsébet Posta Katalin (1961-) (biológia-kémia szakos tanár) Hidvégi Norbert (1986-)
Internet cím:DOI
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001-es BibID:BIBFORM080706
Első szerző:Gulyás Andrea
Cím:Analysis of long term in vitro propagated methylome in apple / Andrea Gulyás, Norbert Hidvégi, Judit Dobránszki, Katalin Posta, Erzsébet Kiss
ISBN:978 963 315 370 3
Tárgyszavak:Természettudományok Biológiai tudományok előadáskivonat
Megjelenés:Fiatal Biotechnológusok Országos Konferenciája / szerk. Tamás László, Zelenyánszki Helga. - p. 119. -
További szerzők:Hidvégi Norbert (1986-) Dobránszki Judit (1967-) (biológus) Posta Katalin (1961-) (biológia-kémia szakos tanár) Kiss Erzsébet
Internet cím:Intézményi repozitóriumban (DEA) tárolt változat


001-es BibID:BIBFORM079121
Első szerző:Gulyás Andrea
Cím:Changes in DNA methylation pattern of apple long-term in vitro shoot culture and acclimatized plants / Andrea Gulyás, Judit Dobránszki, Erzsébet Kiss, Jaime A. Teixeira da Silva, Katalin Posta, Norbert Hidvég
Megjegyzések:DNA methylation is a process of epigenetic modification that can alter the functionality of a genome. Using whole-genome bisulfite sequencing, this study quantify the level of DNA methylation in the epigenomes of two diploid apple (Malus x domestica) scion cultivars ('McIntosh' and 'Húsvéti rozmaring') derived from three environmental conditions: in vivo mother plants in an orchard, in vitro culture, and acclimatized in vitro plants. The global DNA methylation levels were not dependent on the source of plant material, and the average level of DNA methylation was 49.77%, 34.65% and 8.77% in CpG, CHG and CHH contexts, respectively. Significant differences in DNA methylation were identified in 586 (specifically 334, 201 and 131 in CpG, CHG and CHH contexts, respectively) out of 45,116 genes, including promoter and coding sequences. These were classified as differentially methylated genes (DMGs). This is a 1.3% difference in the level of DNA methylation of genes in response to a change in the environment. Differential methylation was visualised by MA plots and functional genomic maps were established for biological processes, molecular functions and cellular components. When the DMGs were considered, in vitro tissue culture resulted in the highest level of methylation, but it was lower in acclimatized in vitro plants which was similar to that in the mother tree. Methylation patterns of the two scions differed, indicating cultivar-specific epigenetic regulation of gene expression during adaptation to various environments. After selecting genes that displayed differences larger than ?10% in CpG and CHG contexts, or larger than ?1.35% in the CHH context from among the DMGs, they were annotated in Blast2?GO v5.1.12 for Gene Ontology. DMGs identified as MD07G1113000 (protein transport), MD08G1041600 (extracellular space), MD09G1054800 (phosphatidic acid binding), and MD10G1265800 (not annotated) were methylated in all three contexts in in vitro shoots. These DNA methylation results suggest that epigenetic changes may contribute to the adaptation of apple to environmental changes by modifying the epigenome and thereby gene expression.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Malus sp.
Tissue culture
Whole-genome bisulfite sequencing
Megjelenés:Journal Of Plant Physiology. - 239 (2019), p. 18-27. -
További szerzők:Dobránszki Judit (1967-) (biológus) Kiss Erzsébet Teixeira da Silva, Jaime A. Posta Katalin (1961-) (biológia-kémia szakos tanár) Hidvégi Norbert (1986-)
Pályázati támogatás:20428-3/2018/FEKUTSTRAT
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001-es BibID:BIBFORM104321
Első szerző:Hidvégi Norbert
Cím:Complementation of wild strawberry (Fragaria vesca L.) SPATULA (FvSPT) and SPIRAL (FvSPR) genes in Arabidopsis thaliana / Norbert Hidvégi, Andrea Gulyás Andrea, Jaime A Teixeira da Silva, Katalin Posta Katalin, Erzsébet Kiss
ISSN:2064-7816 2064-9479
Megjegyzések:This study assessed the function of genes involved in wild strawberry (Fragaria vescaL.) fruitdevelopment and maturation to better understand the mechanism of non-climacteric fruit ripening.SPATULA(FvSPT) andSPIRAL(FvSPR) genes ofFragaria vescadisplayed differential expression between the greenand red ripening stages.SPT, which encodes a bHLH transcription factor, was characterized inArabidopsisthalianaL. where its recessive mutation caused degenerative carpel and fruit development. ThesptmutantofA. thalianahad shorter, smaller, and wider spatula-shaped siliques than the wild type.SPTwas expressedthroughout the development of marginal and transmission tract tissues, confirming its role in regulating thegrowth of these tissues. TwoA. thaliana SPIRALgenes,SPR1andSPR2, are required for directional controlof cell elongation. Recessive mutations in either of these genes decreased anisotropic growth of endodermaland cortical root cells and etiolated hypocotyls and caused right-handed helical growth in epidermal cells.The strawberrySPATULA(FvSPT) andSPIRAL(FvSPR) genes were amplified andsptandsprmutantA.thalianaplants were transformed withFvSPT::pGWB401,FvSPR1-1::pGWB401andFvSPR1-2::pGWB401vector constructs. Silique length and seed number/silique in theA. thaliana sptmutant were effectively com-plemented byFvSPTwhereassprwas almost fully complemented byFvSPR1-2, but not byFvSPR1-1.
Tárgyszavak:Agrártudományok Növénytermesztési és kertészeti tudományok idegen nyelvű folyóiratközlemény hazai lapban
spatula shape-silique
helical root growth
Megjelenés:Columella. - 7 : 1 (2020), p. 23-34. -
További szerzők:Gulyás Andrea (1985-) Teixeira da Silva, Jaime A. Posta Katalin (1961-) (biológia-kémia szakos tanár) Kiss Erzsébet
Pályázati támogatás:NKFIH-1150-6/2019
OTKA- K-101195
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