Összesen 2 találat.


001-es BibID:BIBFORM080015
035-os BibID:(cikkazonosító)126298
Első szerző:Imre Alexandra (1992-)
Cím:A new, rapid multiplex PCR method identifies frequent probiotic origin among clinical Saccharomyces isolates / Imre Alexandra, Rácz Hanna Viktória, Antunovics Zsuzsa, Rádai Zoltán, Kovács Renátó, Lopandic Ksenija, Pócsi István, Pfliegler Walter P.
Megjegyzések:An increasing number of infections originating from probiotic use are reported worldwide, with the majority of such cases caused by the yeast Saccharomyces 'boulardii', a subtype of S. cerevisiae. Reliably linking infectious cases to probiotic products requires unequivocal genotyping data, however, these techniques are often timeconsuming and difficult to implement in routine diagnostics. This leads to a widespread lack of genetic data regarding the origin of Saccharomyces infections. We propose a quick and reliable PCR-based protocol for the identification of S. 'boulardii' based on a combined analysis of interdelta fingerprinting and microsatellite typing. By applying various typing methods and our proposed method to the clinical yeast collection of a Hungarian hospital we show that probiotic origin is common among clinical Saccharomyces, and that the new multiplex method enables rapid and unequivocal identification of probiotic yeast infections. This method can be applied for the identification of yeast infection sources, helping decisions on probiotic use.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Saccharomyces boulardii
Mycological typing techniques
Megjelenés:Microbiological Research. - 227 (2019), p. 1-7. -
További szerzők:Rácz Hanna Viktória (1994-) (biológus) Antunovics Zsuzsa (1978-) (molekuláris biológus, genetikus) Rádai Zoltán (1991-) (biológus) Kovács Renátó László (1987-) (molekuláris biológus) Lopandic, Ksenija Pócsi István (1961-) (vegyész) Pfliegler Valter Péter (1986-) (molekuláris biológus)
Pályázati támogatás:ÚNKP-18-3-I-DE-4
AÖU 98öu7
Internet cím:Szerző által megadott URL
Intézményi repozitóriumban (DEA) tárolt változat


001-es BibID:BIBFORM093483
035-os BibID:(Scopus)85104748956 (WOS)000647236400001
Első szerző:Rácz Hanna Viktória (biológus)
Cím:How to characterize a strain? Clonal heterogeneity in industrial influences both phenotypes and heterogeneity in phenotypes / Hanna Viktória Rácz, Fezan Mukhtar, Alexandra Imre, Zoltán Rádai, Andreas Károly Gombert, Tamás Rátonyi, János Nagy, István Pócsi, Walter P. Pfliegler
Megjegyzések:Populations of microbes are constantly evolving heterogeneity that selection acts upon, yet heterogeneity is non?trivial to assess methodologically. The necessary practice of isolating single cell colonies and thus, subclone lineages for establishing, transferring, and using a strain results in single?cell bottlenecks with a generally neglected effect on the characteristics of the strain itself. Here, we present evidence that various subclone lineages for industrial yeasts sequenced for recent genomic studies show considerable differences, ranging from loss?of?heterozygosity to aneuploidies. Subsequently, we assessed whether phenotypic heterogeneity is also observable in industrial yeast, by individually testing subclone lineages obtained from products. Phenotyping of industrial yeast samples and their newly isolated subclones showed that single?cell bottlenecks during isolation can indeed considerably influence the observable phenotype. Next, we decoupled fitness distributions on the level of individual cells from clonal interference by plating single cell colonies and quantifying colony area distributions. We describe and apply an approach using statistical modeling to compare the heterogeneity in phenotypes accross samples and subclone lineages. One strain was further used to show how individual subclonal lineages are remarkably different not just in phenotype, but also in the level of heterogeneity in phenotype. With these observations, we call attention to the fact that choosing an initial clonal lineage from an industrial yeast strain may vastly influence downstream performances and observations on karyotype, phenotype, and also on heterogeneity.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Fermented foods and beverages
genomic structural variation
phenotypic variability
Saccharomyces boulardii
Megjelenés:Yeast. - 38 : 8 (2021), p. 453-470. -
További szerzők:Mukhtar, Fezan Imre Alexandra (1992-) (biomérnök, biotechnológus) Rádai Zoltán (1991-) (biológus) Gombert Andreas Károly Rátonyi Tamás (1967-) (agrármérnök) Nagy János (1951-) (agrármérnök, mérnök-tanár) Pócsi István (1961-) (vegyész) Pfliegler Valter Péter (1986-) (molekuláris biológus)
Pályázati támogatás:GINOP-2.2.1-15-2016-00001
Internet cím:Szerző által megadott URL
Intézményi repozitóriumban (DEA) tárolt változat
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