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001-es BibID:	BIBFORM096051
035-os BibID:	(WoS)000676122000001 (Scopus)85111301797
Első szerző:	Arianti, Rini (biokémikus)
Cím:	ASC-1 transporter-dependent amino acid uptake is required for the efficient thermogenic response of human adipocytes to adrenergic stimulation / Rini Arianti, Boglarka Agnes Vinnai, Beata B. Toth, Abhirup Shaw, Eva Csosz, Attila Vamos, Ferenc Gyory, Pamela Fischer-Posovszky, Martin Wabitsch, Endre Kristof, Laszlo Fesus
Dátum:	2021
ISSN:	0014-5793
Megjegyzések:	Brown and beige adipocytes dissipate energy by uncoupling protein 1 (UCP1)-dependent and UCP1-independent thermogenesis, which may be utilized to develop treatments against obesity. We have found that mRNA and protein expression of the alanine/serine/cysteine transporter-1 (ASC-1) was induced during adipocyte differentiation of human brown-prone deep neck and beige-competent subcutaneous neck progenitors, and SGBS preadipocytes. cAMP stimulation of differentiated adipocytes led to elevated uptake of serine, cysteine, and glycine, in parallel with increased oxygen consumption, augmented UCP1-dependent proton leak, increased creatine-driven substrate cycle-coupled respiration, and upregulation of thermogenesis marker genes and several respiratory complex subunits; these outcomes were impeded in the presence of the specific ASC-1 inhibitor, BMS-466442. Our data suggest that ASC-1-dependent consumption of serine, cysteine, and glycine is required for efficient thermogenic stimulation of human adipocytes.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk adipocytes ASC-1 inhibition gene expression obesity proton leak respiration thermogenesis uncoupling protein
Megjelenés:	Febs Letters. - 595 : 16 (2021), p. 2085-2098. -
További szerzők:	Vinnai Boglárka Ágnes (1996-) (molekuláris biológus) Bartáné Tóth Beáta (1970-) (molekuláris biológus) Shaw, Abhirup (1992-) Csősz Éva (1977-) (biokémikus, molekuláris biológus) Vámos Attila (1991-) (gyógyszer-biotechnológus) Győry Ferenc (1964-) (sebész) Fischer-Posovszky Pamela Wabitsch, Martin Kristóf Endre (1987-) (általános orvos) Fésüs László (1947-) (orvos biokémikus)
Pályázati támogatás:	GINOP-2.3.2-15-2016-00006 GINOP EFOP-3.6.3-VEKOP-16-2017-00009 EFOP FK131424 OTKA K129139 OTKA ÚNKP-20-5-DE-12 Egyéb ÚNKP-20-2-I-DE-187 Egyéb
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001-es BibID:	BIBFORM127645
035-os BibID:	(scopus)85216862080 (WoS)001423742600001
Első szerző:	Vinnai Boglárka Ágnes (molekuláris biológus)
Cím:	The importance of thiamine availability in the thermogenic competency of human adipocytes / Vinnai Boglárka Ágnes, Arianti Rini, Fischer-Posovszky Pamela, Wabitsch Martin, Fésüs László, Kristóf Endre
Dátum:	2025
ISSN:	0303-7207
Megjegyzések:	Brown and beige adipocytes express uncoupling protein 1 (UCP1), which is located in the inner mitochondrial membrane and facilitates the dissipation of excess energy as heat. The activation of thermogenic adipocytes is a potential therapeutic target for treating type 2 diabetes mellitus, obesity, and related co-morbidities. Therefore, identifying novel approaches to stimulate the function of these adipocytes is crucial for advancing therapeutic strategies. Currently, there are limited amount of human adipocyte cell line models available to study the regulatory mechanisms of browning and key players in thermogenesis. The Simpson-Golabi-Behmel syndrome (SGBS) preadipocyte cell line has been proven as a valuable model to investigate human adipocyte biology. In this study, we investigated how excess thiamine (vitamin B1), and the inhibition of thiamine transporters affect the expression of thermogenic markers and functional parameters during adrenergic stimulation in SGBS adipocytes. We found that limiting thiamine availability by pharmacological inhibitors impeded the dibutyryl-cAMP (db-cAMP)-dependent induction of thiamine transporter 1 and 2 (encoded by SLC19A2 and SLC19A3), UCP1, PGC1a, and other browning markers, as well as proton leak respiration which is associated with UCP1-dependent heat generation. Contrarily, excess thiamine enhanced the db-cAMP-dependent induction of thiamine transporters, while UCP1, PGC1a, and other browning markers were upregulated. In addition, abundant amounts of thiamine increased the basal, unstimulated coupled and uncoupled respiration, and the expression of mitochondrial complex subunits. Our study highlights the critical role of excess thiamine in the thermogenic activation of SGBS adipocytes and its potential to enhance thermogenesis.
Tárgyszavak:	Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban folyóiratcikk Obesity Adipocytes Thermogenesis Thiamine UCP1 SGBS
Megjelenés:	Molecular And Cellular Endocrinology. - 599 (2025), p. 1-14. -
További szerzők:	Arianti, Rini (1991-) (biokémikus) Fischer-Posovszky Pamela Wabitsch, Martin Fésüs László (1947-) (orvos biokémikus) Kristóf Endre (1987-) (általános orvos)
Pályázati támogatás:	FK145866 OTKA PD146202 OTKA EKÖP-24-3-II-DE-113 Egyéb
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