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001-es BibID:BIBFORM110227
035-os BibID:(cikkazonosító)6216 (Scopus)85152336402 (WoS)000969756600001
Első szerző:Cozzolino, Marco (biológus)
Cím:The Voltage-Gated Hv1 H⁺ Channel Is Expressed in Tumor-Infiltrating Myeloid-Derived Suppressor Cells / Cozzolino Marco, Gyöngyösi Adrienn, Korpos Eva, Gogolak Peter, Naseem Muhammad Umair, Kállai Judit, Lanyi Arpad, Panyi Gyorgy
Megjegyzések:Myeloid-derived suppressor cells (MDSCs) are key determinants of the immunosuppressive microenvironment in tumors. As ion channels play key roles in the physiology/pathophysiology of immune cells, we aimed at studying the ion channel repertoire in tumor-derived polymorphonuclear (PMN-MDSC) and monocytic (Mo-MDSC) MDSCs. Subcutaneous tumors in mice were induced by the Lewis lung carcinoma cell line (LLC). The presence of PMN-MDSC (CD11b+/Ly6G+) and Mo-MDSCs (CD11b+/Ly6C+) in the tumor tissue was confirmed using immunofluorescence microscopy and cells were identified as CD11b+/Ly6G+ PMN-MDSCs and CD11b+/Ly6C+/F4/80?/MHCII? Mo-MDSCs using flow cytometry and sorting. The majority of the myeloid cells infiltrating the LLC tumors were PMN-MDSC (~60%) as compared to ~10% being Mo-MDSCs. We showed that PMN- and Mo-MDSCs express the Hv1 H+ channel both at the mRNA and at the protein level and that the biophysical and pharmacological properties of the whole-cell currents recapitulate the hallmarks of Hv1 currents: ~40 mV shift in the activation threshold of the current per unit change in the extracellular pH, high H+ selectivity, and sensitivity to the Hv1 inhibitor ClGBI. As MDSCs exert immunosuppression mainly by producing reactive oxygen species which is coupled to Hv1-mediated H+ currents, Hv1 might be an attractive target for inhibition of MDSCs in tumors.
Tárgyszavak:Orvostudományok Elméleti orvostudományok idegen nyelvű folyóiratközlemény külföldi lapban
Myeloid-derived suppressor cell
Hv1 proton channel
tumor microenvironment
Megjelenés:International Journal Of Molecular Sciences. - 24 : 7 (2023), p. 1-24. -
További szerzők:Gyöngyösi Adrienn (1982-) (biológus) Korpos Éva (1974-) Gogolák Péter (1968-) (biológus, immunológus) Naseem, Muhammad Umair (1993-) (biofizikus, molekuláris biológus) Kállai Judit (1983-) (molekuláris biológus) Lányi Árpád (1962-) (biológus, immunológus) Panyi György (1966-) (biofizikus)
Pályázati támogatás:K119417
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