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001-es BibID:BIBFORM071242
Első szerző:Pataki Emese (molekuláris biológus)
Cím:fhl1 controls transcription of the nitrogen starvation induced genes in cooperation with TORC1 in Schizosaccharomyces pombe / Emese Pataki, Ronit Weisman, Mátyás Sipiczki, Ida Miklós
ISSN:1217-8950 1588-2640
Megjegyzések:One of the most important regulators of cellular processes is the protein kinase TOR (Target ofRapamycin), which is conserved from yeast to humans. TOR is a protein kinase that was isolated asthe target of the immunosuppressive and anticancer drug rapamycin. It regulates the response toenvironmental changes and cellular energy status, cell growth, cell proliferation, cell survival,protein synthesis, and transcription. In the fission yeast, Schizosaccharomyces pombe there are twoTOR homologs. Tor1 is part of a protein complex known as TORC2, while Tor2 is part of a proteincomplex known as TORC1. These complexes were found to regulate oppositely the response tonutrient depletion, in particular the response to nitrogen starvation. TORC1 is essential and playsmajor role in the control of cellular growth, while TORC2 regulates cell survival under stressconditions. Here we demonstrate that overexpression of fhl1+ gene suppresses the mRNA level ofthe nitrogen starvation response genes: mei2, ste4, ste11 and decreases the mating efficiency of tor2-ts mutant. Moreover overexpression of fhl1+ modifies the stress response of tor2-ts mutant, amongothers in the presence of rapamycin.Based on these findings we can conclude that fhl1 plays a role in the TOR pathway and tor2controls the response to nitrogen starvation via fhl1 transcription factor.
Tárgyszavak:Természettudományok Biológiai tudományok idézhető absztrakt
Megjelenés:Acta Microbiologica et Immunologica Hungarica 62 : Suppl. (2015), p. 197-197. -
További szerzők:Weisman, Ronit Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
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001-es BibID:BIBFORM070493
Első szerző:Pataki Emese (molekuláris biológus)
Cím:fhl1 gene of the fission yeast regulates transcription of meiotic genes and nitrogen starvation response, downstream of the TORC1 pathway / Emese Pataki, Ronit Weisman, Matthias Sipiczki, Ida Miklos
ISSN:0172-8083 1432-0983
Megjegyzések:Environmental changes, such as nutrient limitation or starvation induce different signal transducing pathways, which require coordinated cooperation of several genes. Our previous data revealed that the fhl1 fork-head type transcription factor of the fission yeast could be involved in sporulation, which was typically induced under poor conditions. Since the exact role of Fhl1 in this process was not known, we wanted to identify its downstream targets and to investigate its possible cooperation with another known regulator of sporulation. Gene expression and Northern blot analysis of the fhl1? mutant strain revealed the target genes involved in mating and sporulation. Our results also showed that Fhl1 could regulate nutrient sensing, the transporter and permease genes. Since the majority of these genes belonged to the nitrogen starvation response, the possible cooperation of fhl1 and tor2 was also investigated. Comparison of their microarray data and the expression of fhl1 + from a strong promoter in the tor2-ts mutant cells suggested that one part of the target genes are commonly regulated by Fhl1 and Tor2. Since the expression of fhl1 + from a strong promoter could rescue rapamycin and temperature sensitivity and suppressed the hyper-sporulation defect of the tor2-ts mutant cells, we believe that Fhl1 acts in TOR signaling, downstream of Tor2. Thus, this work shed light on certain novel details of the regulation of the sexual processes and a new member of the TOR pathway, but further experiments are needed to confirm the involvement of Fhl1 in nutrient sensing.
Tárgyszavak:Természettudományok Biológiai tudományok idegen nyelvű folyóiratközlemény külföldi lapban
Schizosaccharomyces pombe
TOR pathway
Fork-head transcription factor
nitrogen response
Megjelenés:Current Genetics 63 : 1 (2016), p. 91-101. -
További szerzők:Weisman, Ronit Sipiczki Mátyás (1948-) (biológus) Miklós Ida (1962-) (biológus)
Pályázati támogatás:OTKA-106172
TÁMOP 4.2.2.B-15/1/KONV-2015-0001.
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